Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics
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TLDR
No significant differences were observed in first or second cell-cycle length, synchrony of the second or third cell cycles, duration of blastulation, multinucleation at the 2-cell stage and irregular division patterns between euploid and aneuploid embryos.Abstract:
This study determined whether morphokinetic variables between aneuploid and euploid embryos differ as a potential aid to select euploid embryos for transfer. Following insemination, EmbryoScope time-lapse images from 98 blastocysts were collected and analysed blinded to ploidy. The morphokinetic variables were retrospectively compared with ploidy, which was determined fol- lowing trophectoderm biopsy and analysis by array comparative genomic hybridization or single-nucleotide polymorphic array. Multiple aneuploid embryos were delayed at the initiation of compaction (tSC; median 85.1 hours post insemination (hpi); P = 0.02) and the time to reach full blastocyst stage (tB; median 110.9 hpi, P = 0.01) compared with euploid embryos (tSC median 79.7 hpi, tB median 105.9 hpi). Embryos having single or multiple aneuploidy (median 103.4 hpi, P = 0.004 and 101.9 hpi, P = 0.006, respec- tively) had delayed initiation of blastulation compared with euploid embryos (median 95.1 hpi). No significant differences were observed in first or second cell-cycle length, synchrony of the second or third cell cycles, duration of blastulation, multinucleation at the 2-cell stage and irregular division patterns between euploid and aneuploid embryos. This non-invasive model for ploidy clas- sification may be used to avoid selecting embryos with high risk of aneuploidy while selecting those with reduced risk. RBMOnlineread more
Citations
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Embryo morphokinetic characteristics and euploidy.
TL;DR: Time lapse technology has allowed us to obtain a lot of information about human embryo development through the characterization of events that are otherwise not visible using static morphological observations.
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Blastocyst score, a blastocyst quality ranking tool, is a predictor of blastocyst ploidy and implantation potential.
TL;DR: The BS is a predictor of BL ploidy and FH implantation and can stratify BL into good (3–5), fair (6–9), and poor (10–14) groups, reflecting their FH, live birth rates, and ploidsy status.
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The unknown human trophectoderm: implication for biopsy at the blastocyst stage
TL;DR: It is suggested that the trophectoderm origin and functions have been insufficiently ascertained in humans so far and should be considered an experimental procedure to be undertaken only under approved rigorous experimental protocols in academic contexts.
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Non-invasive methods for embryo selection.
TL;DR: A review of the proposed non-invasive methods for embryo selection attempts to evaluate them in the light of the best currently available evidence and to find out whether any of them is ripe for replacing or supplementing the time-honored method of morphological assessment.
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Contraction behaviour reduces embryo competence in high-quality euploid blastocysts
Xavier Viñals Gonzalez,Rabi Odia,Suzanne Cawood,Matthew Gaunt,Wael Saab,S Seshadri,Paul Serhal +6 more
TL;DR: It becomes clear that embryo contraction, despite being a physiological feature during blastulation, is conditioned by the ploidy status of the embryo, and the presence of contractions may compromise implantation rates.
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