Molecular Epidemiologic Characterization of Enterocytozoon bieneusi in HIV-Infected Persons in Benin City, Nigeria
Frederick Olusegun Akinbo,Christopher E Okaka,Richard Omoregie,Theressa Dearen,Eucaris Torres Leon,Lihua Xiao +5 more
TLDR
Six known genotypes of E. bieneusi, including D, A, IV, CAF2, EbpA, and Peru 8 in 31, 22, 14, 2, 1, and 1 patients, respectively, five novel genotypes, and one infection with mixed genotypes were observed in this study.Abstract:
Molecular characterization of Enterocytozoon bieneusi has led to better understanding of microsporidiosis transmission in humans. This study aimed to detect and genotype E. bieneusi in human immunodeficiency virus (HIV)-infected persons. Stool specimens were collected from 463 HIV-infected patients and analyzed for E. bieneusi by polymerase chain reaction (PCR) and DNA sequence analysis of the internal transcribed spacer. E. bieneusi was detected in 77 HIV patients. CD4 cell counts < 200 cells/μL was associated with E. bieneusi infection (P = 0.09). E. bieneusi was significantly associated with weight loss (P < 0.0001), diarrhea (P = 0.006), fever (P < 0.0001), not being married (P < 0.0001), and flush type of toilet (P = 0.0007). Six known genotypes of D, A, IV, CAF2, EbpA, and Peru 8 in 31, 22, 14, 2, 1, and 1 patients, respectively, five novel genotypes of E. bieneusi, and one infection with mixed genotypes were observed in this study. Three of the novel genotypes were genetically distant to the genotypes commonly found in humans.read more
Citations
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Book ChapterDOI
Microsporidia and ‘The Art of Living Together’
Jiří Vávra,Julius Lukeš +1 more
TL;DR: This review attempts to present a general overview of microsporidia, emphasising some less known and/or more recently discovered facets of their biology.
Journal ArticleDOI
Zoonotic Cryptosporidium species and Enterocytozoon bieneusi genotypes in HIV-positive patients on antiretroviral therapy
Lin Wang,Hongwei Zhang,Xudong Zhao,Longxian Zhang,Guoqing Zhang,Meijin Guo,Lili Liu,Yaoyu Feng,Lihua Xiao +8 more
TL;DR: Wang et al. as mentioned in this paper examined the distribution of Cryptosporidium species and Enterocytozoon bieneusi genotypes in AIDS patients receiving antiretroviral therapy.
Journal ArticleDOI
Molecular Surveillance of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi by Genotyping and Subtyping Parasites in Wastewater
Na Li,Lihua Xiao,Lin Wang,Shuming Zhao,Xukun Zhao,Liping Duan,Meijin Guo,Lili Liu,Yaoyu Feng +8 more
TL;DR: The distribution of Cryptosporidium species, G. duodenalis genotypes and subtypes, and E. bieneusi genotypes in urban wastewater indicates that anthroponotic transmission appeared to be important in epidemiology of cryptosporidiosis, giardiasis, and microsporidia in the study areas.
Journal ArticleDOI
Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections
TL;DR: This review provides an overview of the multitude of methods that have been reported for the detection of intestinal parasites and offers some guidance in applying these methods in the clinical laboratory and in epidemiological studies.
Journal ArticleDOI
Host Specificity of Enterocytozoon bieneusi and Public Health Implications.
Wei Li,Yaoyu Feng,Mónica Santín +2 more
TL;DR: This review summarizes all E. bieneusi genotypes identified thus far based on sequence analysis of the ribosomal internal transcribed spacer from specimens obtained from humans, domestic and wild animals, and water sources and interprets the public health significance of genotype groups and major zoonotic genotypes with the goal of improving understanding of host specificity.
References
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Journal ArticleDOI
Zoonotic Potential of the Microsporidia
TL;DR: Vertebrate hosts are now identified for all four major microsporidial species infecting humans (E. bieneusi and the three Encephalitozoon spp.), implying a zoonotic nature of these parasites.
Journal ArticleDOI
Microsporidiosis: Enterocytozoon bieneusi in domesticated and wild animals.
Mónica Santín,Ronald Fayer +1 more
TL;DR: The information presented in this review should be useful in understanding the taxonomy, epidemiology, zoonotic potential, and importance in public health of E. bieneusi.
Journal ArticleDOI
Enterocytozoon bieneusi genotype nomenclature based on the internal transcribed spacer sequence: a consensus.
Mónica Santín,Ronald Fayer +1 more
TL;DR: It is proposed that the first published name has precedence and should become the primary name used in all subsequent publications in which genotyping is based on ITS sequencing.
Journal ArticleDOI
Molecular characterization of microsporidia indicates that wild mammals Harbor host-adapted Enterocytozoon spp. as well as human-pathogenic Enterocytozoon bieneusi.
TL;DR: Results of this study indicate that fur-bearing mammals, especially those closely associated with surface water, can be a potential source of human-pathogenic E. bieneusi in wildlife and there are also host-adapted Enterocytozoon genotypes in wildlife, which may represent species different from E.Bienesi.
Journal ArticleDOI
Enterocytozoon bieneusi in human and animals, focus on laboratory identification and molecular epidemiology.
Marc Thellier,J Breton +1 more
TL;DR: Molecular analyses of the 243-bp internal Transcribed spacer-(ITS) of the rRNA gene have revealed a considerable genetic variation within E. bieneusi isolates of human and animal origins, supporting the potential for zoonotic transmission.
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