Morphological aspects of the uptake of simian virus 40 by permissive cells.
Klaus Hummeler,Klaus Hummeler,Natale Tomassini,Natale Tomassini,Frantisek Sokol,Frantisek Sokol +5 more
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After exposure of permissive cells to simian virus 40 (SV40), single particles were engulfed by the cell membrane and transported to the nucleus and none of the original size were found in the nucleus 1 hr after infection.Abstract:
After exposure of permissive cells to simian virus 40 (SV40), single particles were engulfed by the cell membrane and transported to the nucleus. The cell membrane closed tightly around the particles, increasing their diameter from 40 to 55 nm. The cell membrane was lost during interaction with the nuclear membranes, and particles of the original size were found in the nucleus 1 hr after infection. Uncoating of these nuclear particles occurred rapidly, and none could be found 4 hr after infection. Viral progeny appeared 24 hr after infection.read more
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Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER.
TL;DR: The results demonstrate the existence of a two-step transport pathway from plasma-membrane caveolae, through an intermediate organelle (termed the caveosome), to the ER, which bypasses endosomes and the Golgi complex, and is part of the productive infectious route used by SV40.
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Integration of murine leukemia virus DNA depends on mitosis
TL;DR: Viral nucleoprotein complexes isolated from arrested cells contain full‐length viral DNA and can integrate this viral DNA in vitro, showing that the block to integration in arrested cells is not due to a lack of mature integration machinery.
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Virus entry by endocytosis.
TL;DR: This review focuses on the cell biology of virus entry and the different strategies and endocytic mechanisms used by animal viruses.
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Endocytosis via caveolae.
Lucas Pelkmans,Ari Helenius +1 more
TL;DR: Caveolae are flask‐shaped invaginations present in the plasma membrane of many cell types and are directly involved in the internalization of membrane components, extracellular ligands, and several nonenveloped viruses.
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Virus entry into animal cells.
Mark Marsh,Ari Helenius +1 more
TL;DR: In order to take up nutrients, to communicate with other cells, to control the intracellular ion balance, and to secrete substances, cells have a variety of mechanisms for bypassing and modifying the barrier properties imposed by their plasma membrane.
References
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Infection of human and simian tissue cultures with rous sarcoma virus
TL;DR: The interfacial phenomena described here are somewhat similar to the "interfacial turbulence" observed by chemical engineers during the process of solvent extraction, but the mechanisms are quite different.
Journal ArticleDOI
An electron microscope study of the development of SV40 virus.
TL;DR: Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus and various nuclear and cytoplasmic lesions in relation to the infection are described.
Journal ArticleDOI
Studies on DNA from low-density particles of SV40: I. Heterogeneous defective virions produced by successive undiluted passages
TL;DR: Successive undiluted passages of SV40 (small plaque) in African green monkey kidney cells result in the production of heterogeneous defective particles of lower densities than that of infectious virions, which would account for defectiveness as well as alteration in density of virions.
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The purification of simian virus 40
TL;DR: A procedure for the purification of simian virus 40 involved treatment of the pellet obtained by centrifugation of the crude virus suspension at 80,000 g with trypsin and deoxycholate, followed by centrifugalation in a density gradient.
Journal ArticleDOI
Structure of simian virus 40. II. Symmetry and components of the virus particle.
TL;DR: Evidence is presented that the three types of shells of smaller size are derived from reassembled morphological units of normal particle shells based on Examination of the surface structure of the smaller particles strongly suggested icosahedral surface lattices of T =3, T = 3, and T = 4 symmetry classes.