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Multistep Engineering of a Penicillin G Acylase for Systematic Improvement of Crystallization Efficiency

Janine R. Mayer, +3 more
- 05 Apr 2023 - 
- Vol. 23, Iss: 5, pp 3230-3243
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This article is published in Crystal Growth & Design.The article was published on 2023-04-05 and is currently open access. It has received 0 citations till now. The article focuses on the topics: Crystallization & Penicillin amidase.

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TL;DR: Coot is a molecular-graphics program designed to assist in the building of protein and other macromolecular models and the current state of development and available features are presented.
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Phaser crystallographic software

TL;DR: A description is given of Phaser-2.1: software for phasing macromolecular crystal structures by molecular replacement and single-wavelength anomalous dispersion phasing.
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TL;DR: A new method, based on chemical thermodynamics, is developed for automatic detection of macromolecular assemblies in the Protein Data Bank (PDB) entries that are the results of X-ray diffraction experiments, as found, biological units may be recovered at 80-90% success rate, which makesX-ray crystallography an important source of experimental data on macromolescular complexes and protein-protein interactions.
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