Showing papers in "Immunochemistry in 1976"

TL;DR: The cationic polypeptide polymyxin B, has been demonstrated to form a stable molecular complex with the lipid A region of bacterial lipopolysaccharides, which results in the generation of higher mol.

TL;DR: The cross-reaction between avian and human fibronectin suggests that fibronECTin has remained highly conserved in evolution and may be to do its functions as cell surface protein.

TL;DR: Correlation of complementarity region heavy chain sequences of several mouse phosphorylcholine-binding immunoglobulins with the known three-dimensional structure of the hapten binding cavity in one of them (M603) reveals the physical-chemical nature of the interaction between protein and ligand.

TL;DR: The inhibition of C 1 was shown to be the result of a direct interaction of at least liquoid, dextran sulfate, polyvinyl sulfate and heparin with the C1 subunit Clq: preincubation of these substances with purified Clq reduced their inhibitory capacity against free C 1.

TL;DR: These specific haemolytic assays have been extended to the detection of the factors following isoelectric focusing and allows the heterogeneity of the components to be investigated in unfractionated serum.

TL;DR: The process of this invention can be used to determine the concentration of any substance capable of specifically promoting or inhibiting an agglutination reaction such as viruses, white blood cells or the like.

TL;DR: The inhibition of C2 uptake by polyanion could be reduced by increasing magnesium concentration to levels which are normally inhibitory, suggesting that Mg ++ ions are sequestered by these sulfated polyanions.

TL;DR: In contrast to C4 and C2, PA had no effect on the esterolytic activity of C1s against the synthetic substrate TAMe, indicating that PA do not interfere with the enzymatic site of C 1s.

TL;DR: Findings support the hypothesis that immunological mechanisms are involved in the suppressive phenomenon of experimental allergic encephalomyelitis by synthetic basic copolymers.

TL;DR: From such cross reactions, the focus of his experimental work during his last three decades, Heidelberger learned many details about the chemical composition of antigens and antibodies.

TL;DR: In this article, statistical analyses of the carbohydrate compositions of eleven IgA1 and six IgA2 meyloma proteins revealed that in addition to the known difference in the content of galactosamine, which is present only in proteins of the IgA 1 subclass, there are further quantitative differences in the carbohydrate composition of both subclasses.

TL;DR: Salt-mediated hydrophobic chromatography, a technique by which hydrophilic interactions of a protein with a given ligand coupled to Sepharose are enhanced by specific salts, was used for human IgA purification.

TL;DR: The results suggest that membrane immunoglobulins of T and B lymphocytes are structurally distinct and that T cell Ig may be more hydrophobic than its B cell counterpart.

TL;DR: T cell receptors for antigen may be either wholly or partly composed of glycosyl transferases, which evolved from those involved in the production of histocompatibility antigens and associated mechanisms of cellular interaction and communication present in very primitive organisms.

TL;DR: It was concluded that the enhanced interaction of this RF with IgG that has been aggregated is due entirely to multiple bonds between individual antigen and antibofy molecules.

TL;DR: Binding experiments with tobacco mosaic virus and IgG antibody were analysed by two types of Scatchard plot and association constants calculated in the region where IgG binds univalently were considerably lower than for bivalent binding.

TL;DR: Fluorescein was judged to approximate a complete antigenic group, and the amino acid enrichment of the ligand, to simulate carrier conjugation, had minimal effect on ligand binding.

TL;DR: Evidence is given that the component is an esterase whose irreversible inhibition by the organophosphate reagent, DFP, shows the presence of a serine residue essential for enzymatic activity.

TL;DR: Complement component profiles of human serum treated either IgA or its fragments revealed that the classical pathway of the complement system was activated.

TL;DR: Fractionation of the samples by Sephadex G-200 and DEAE-cellulose chromatography, as well as treatment with 2-mercaptoethanol, indicated that the antibodies belong to both IgG and IgM classes.

TL;DR: This paper showed that chicken anti-IgM antisera contain antibodies directed at the surface immunoglobulin of murine B cells and in addition contain a minor fraction reacting in indirect immunofluorescence with thymocytes, peripheral T-lymphocytes, and thymus-derived leukemia cell lines in culture.

TL;DR: Analysis by polyacrylamide gel electrophoresis showed that the radioactivity precipitated by eluates of anti-IgM treated cell was virtually all associated with IgM, and it is suspected that much of the surface Ig is irreversibly aggregated and that the observed peptides represent oligomers of smaller units.

TL;DR: The IgE-receptor complexes isolated from either cell type eluted at almost identical volumes, and a comparison with polymeric IgA suggested that they had a mol.

TL;DR: A laser light scattering immunoassay was used to measure serim concentrations of IgA in various mouse strains and a qaulitative analysis of the roles of Coulomb repulsion and van der Waals attraction of the latex particles in specific and non-specific agglutination is presented.

TL;DR: The experiments suggest that each of the three anti-galactans binds to each of four galactans from plant, invertebrate and vertebrate origin, suggesting that all threeAnti-Galactans recognize the same or closely related determinant groups.

TL;DR: The B region of cholera enterotoxin is entirely equivalent to choleragenoid, the spontaneously formed enterotoxoid, and the amino acid sequences reported herein differ significantly from those reported previously by others.

TL;DR: The results suggest that the high of occurence of molecules with CRI must reflect the presence of a relatively large number of precursor lymphocytes with the corresponding receptor.

TL;DR: A comparative study has been made of the precipitating and non-precipitating sheep antibodies in the γ 1 and γ 2 immunoglobulin fractions, finding that it is only theγ 1 precipitating antibody that originate a reverse Arthus phenomenon.

TL;DR: Fluorescence quenching titrations of a group of anti-hemoglobin antibodies from a goat immunized with human HbA1 showed the first and the last of these fractions to be directed toward single antigenic sites on the β chain.