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Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3

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TLDR
These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.
Abstract
Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

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Citations
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Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects

TL;DR: A comprehensive view on the mechanism of action, production, applications in the medical field, and health and environmental concerns that are allegedly caused due to these nanoparticles is provided in this paper.
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Biosynthesis of nanoparticles: technological concepts and future applications

TL;DR: In this review, the role of microorganisms and plants in the synthesis of nanoparticles is critically assessed.
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Biological synthesis of metal nanoparticles by microbes.

TL;DR: The current status of microbial synthesis and applications of metal nanoparticles is presented and several factors such as microbial cultivation methods and the extraction techniques have to be optimized and the combinatorial approach such as photobiological methods may be used.
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Synthesis of silver nanoparticles: chemical, physical and biological methods.

TL;DR: An overview of silver nanoparticle preparation by physical, chemical, and biological synthesis is presented to reflect on the current state and future prospects, especially the potentials and limitations of the above mentioned techniques for industries.
Journal ArticleDOI

Biological Synthesis of Nanoparticles from Plants and Microorganisms

TL;DR: The potential uses of various biological sources for nanoparticle synthesis and the application of those nanoparticles are explored and the recent milestones achieved are highlighted by controlling critical parameters, including the choice of biological source, incubation period, pH, and temperature.
References
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Journal ArticleDOI

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis

TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Surface Plasmon Spectroscopy of Nanosized Metal Particles

TL;DR: In this paper, the use of optical measurements to monitor electrochemical changes on the surface of nanosized metal particles is discussed within the Drude model, and the absorption spectrum of a metal sol in water is shown to be strongly affected by cathodic or anodic polarization, chemisorption, metal adatom deposition, and alloying.
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Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum

TL;DR: It is observed that aqueous silver ions when exposed to the fungus Fusarium oxysporum are reduced in solution, thereby leading to the formation of an extremely stable silver hydrosol, creating the possibility of developing a rational, fungal-based method for the synthesis of nanomaterials over a range of chemical compositions, which is currently not possible by other microbe-based methods.
Journal ArticleDOI

Colorimetric Methods Of Analysis

TL;DR: In this article, colorimetric methods of analysis were used for analysis of color data in the context of analysis of the color spectrum of a color-imetric method of analysis.
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