Journal ArticleDOI
Nitrogen fixation in the rhizosphere of rice
Reads0
Chats0
TLDR
The use of a modified assay system is reported to find significant differences in the rates of acetylene reduction associated with the rhizosphere of 50 different strains of rice, which suggests that the rice plant genotype influences the association with nitrogen-fixing bacteria in itsrhizosphere.Abstract:
THE discovery of nitrogen-fixing activity associated with the rhizosphere of non-leguminous plants such as tropical grasses, rice and maize has provoked considerable interest1–9. Discrepancies in the measurements of this nitrogen-fixing activity by the acetylene reduction assay in different conditions prompted us to develop a modified assay system. We report here the use of this assay to find significant differences in the rates of acetylene reduction associated with the rhizosphere of 50 different strains of rice. This suggests that the rice plant genotype influences the association with nitrogen-fixing bacteria in its rhizosphere.read more
Citations
More filters
Journal ArticleDOI
Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences.
TL;DR: The analyses document the presence of eight novel NifH types, which appear to be a variety of significant components of the diazotrophic community, dominated mainly by proteobacteria.
Journal ArticleDOI
Host variation for interactions with beneficial plant-associated microbes
Kevin P. Smith,Robert M. Goodman +1 more
TL;DR: Future research into the role of plant genes involved in hosting beneficial plant-associated microbes will provide greater insight into this relatively unexplored area of biology and should provide new tools to improve plant health in agriculture.
Book ChapterDOI
Biological N2 Fixation in wetland rice fields: Estimation and contribution to nitrogen balance
P. A. Roger,Jagdish K. Ladha +1 more
TL;DR: Improvements and new approaches in methodologies for estimating biological N2 fixation (BNF) in wetland soils are reviewed, earlier quantitative estimates and recent data are summarized, and the contribution of BNF to N balance in Wetland-rice culture is discussed.
Journal ArticleDOI
Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties
Adel Elbeltagy,Kiyo Nishioka,H. Suzuki,Tadashi Sato,Yo Ichiro Sato,Hisao Morisaki,Hisayuki Mitsui,Kiwamu Minamisawa +7 more
TL;DR: Endophytic bacteria were isolated from surface-sterilized stems, seeds, and leaf sheaths of wild and traditionally cultivated rice varieties and various phenotypic traits that are expected to be involved in the persistence and functions of the bacteria were analyzed.
Journal ArticleDOI
Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses.
P van Berkum,B B Bohlool +1 more
TL;DR: This corrects the article on p. 494 in vol.
References
More filters
Journal ArticleDOI
The Acetylene-Ethylene Assay for N2 Fixation: Laboratory and Field Evaluation
TL;DR: This assay was successfully applied to measurements of N(2) fixation by other symbionts and by free living soil microorganisms, and was also used to assess the effects of light and temperature on the N( 2) fixing activity of soybeans.
Journal ArticleDOI
Methods for Growing Spirillum lipoferum and for Counting It in Pure Culture and in Association with Plants.
TL;DR: Methods are described for growing Spirillum lipoferum in quantities sufficient to serve as inoculant in field trials of its associative N(2)-fixing ability with higher plants and as a source of cells for the preparation of nitrogenase, cytochromes, respiratory enzymes, etc.
Journal ArticleDOI
Potential for nitrogen fixation in maize genotypes in Brazil.
TL;DR: periodic sampling within a brachytic maize cultivar revealed that maximum nitrogenase activity occurred at about the 75% silking stage, and N(2)-fixing Spirillum sp.
Journal ArticleDOI
Nitrogenase Activity and Oxygen Sensitivity of the Paspalum notatum-Azotobacter paspali Association
TL;DR: Nitrogenase activity in the rhizosphere of a grass, Paspalum notatum, and its associated soil was measured by the reduction of acetylene, which showed almost completely inhibited in air or in the absence of O2 and was greatest at around pO2 0.04 atm.
Book ChapterDOI
Nitrogen fixation--assay methods and techniques.
TL;DR: The 15 N 2 isotope is a sensitive and specific tracer of N 2 fixation as discussed by the authors, which is a standard method to refer other methods to which other methods should be referred.