Journal ArticleDOI
Non-covalent capillary coatings for protein separations in capillary electrophoresis.
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TLDR
A synopsis of the fundamentals underlying protein adsorption and its impact on CE performance is provided and the efficacy of small molecule background electrolyte additives, surfactants, physically adsorbed polymers (dynamic and static), and successive multiple ionic-polymer layer coatings are evaluated.About:
This article is published in Journal of Chromatography A.The article was published on 2008-03-14. It has received 286 citations till now. The article focuses on the topics: Protein adsorption & Capillary electrophoresis.read more
Citations
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Journal ArticleDOI
Upper Critical Solution Temperature of Poly(N-acryloyl glycinamide) in Water: A Concealed Property
TL;DR: Recently, the nonionic homopolymer poly(N-acryloyl glycinamide) (poly(NAGA)) has been shown to exhibit a sharp uppe... as mentioned in this paper.
Journal ArticleDOI
Relevance and use of capillary coatings in capillary electrophoresis–mass spectrometry
TL;DR: It is concluded that capillary coatings can enhance the performance and stability of CE-MS systems, yielding a highly valuable and reproducible analytical tool.
Journal ArticleDOI
Intact protein analysis in the biopharmaceutical field
Aline Staub,Davy Guillarme,Davy Guillarme,Julie Schappler,Julie Schappler,Jean-Luc Veuthey,Jean-Luc Veuthey,Serge Rudaz,Serge Rudaz +8 more
TL;DR: This review summarizes the analytical techniques available for intact protein determination and the main development steps in which they are applicable and highlights how several analytical strategies are necessary to obtain global information.
Journal ArticleDOI
Protein attachment onto silica surfaces--a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE.
TL;DR: This review addresses the fundamentals governing the adsorption of individual protein molecules onto the surface of fused‐silica capillaries, the protein aggregation to adsorbate clusters and their final accretion to monolayers with subsequent stratification to protein multilayers.
Journal ArticleDOI
Present state of microchip electrophoresis: state of the art and routine applications.
Eric R. Castro,Andreas Manz +1 more
TL;DR: This review will consider the present state of M CE including recent advances in technology and both novel and routine applications in the laboratory and the impact of MCE in the scientific community and its prospects for the future.
References
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Book
Intermolecular and surface forces
TL;DR: The forces between atoms and molecules are discussed in detail in this article, including the van der Waals forces between surfaces, and the forces between particles and surfaces, as well as their interactions with other forces.
Journal ArticleDOI
Theory of self-assembly of hydrocarbon amphiphiles into micelles and bilayers
TL;DR: In this paper, a simple theory is developed that accounts for many of the observed physical properties of micelles, both globular and rod-like, and of bilayer vesicles composed of ionic or zwitterionic amphiphiles.
Book ChapterDOI
Some factors in the interpretation of protein denaturation.
TL;DR: The chapter reviews that the denaturation is a process in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement.
Journal ArticleDOI
Supported Membranes: Scientific and Practical Applications
TL;DR: Supporting lipid-protein bilayers form versatile models of low-dimensionality complex fluids, which can be used to study interfacial forces and wetting phenomena, and enable the design of phantom cells to explore the interplay of lock-and-key forces and universal forces for cell adhesion.