Y
Yasushi Ishihama
Researcher at Kyoto University
Publications - 289
Citations - 24703
Yasushi Ishihama is an academic researcher from Kyoto University. The author has contributed to research in topics: Phosphorylation & Proteome. The author has an hindex of 60, co-authored 260 publications receiving 21169 citations. Previous affiliations of Yasushi Ishihama include Eisai & Osaka Prefecture University.
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Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
TL;DR: StageTips are ordinary pipette tips containing very small disks made of beads with reversed phase, cation-exchange or anion-ex exchange surfaces embedded in a Teflon mesh that allow flexible combination of disks with different surfaces to obtain multi-functional tips.
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Stop and Go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and lc/ms sample pretreatment in proteomics
TL;DR: A novel procedure in which a very small disk of beads embedded in a Teflon meshwork is placed as a microcolumn into pipet tips, finding that the Stage system is well-suited as a universal sample preparation system for proteomics.
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Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein
Yasushi Ishihama,Yoshiya Oda,Tsuyoshi Tabata,Toshitaka Sato,Takeshi Nagasu,Juri Rappsilber,Matthias Mann +6 more
TL;DR: It is reported that PAI values obtained at different concentrations of serum albumin show a linear relationship with the logarithm of protein concentration in LC-MS/MS experiments, and the values of emPAI are suggested that they should be reported in large scale proteomic identification projects.
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Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis
Taishi Umezawa,Naoyuki Sugiyama,Masahide Mizoguchi,Shimpei Hayashi,Fumiyoshi Myouga,Kazuko Yamaguchi-Shinozaki,Yasushi Ishihama,Takashi Hirayama,Kazuo Shinozaki +8 more
TL;DR: It is demonstrated that group A PP2Cs act as ‘gatekeepers’ of subclass III SnRK2s, unraveling an important regulatory mechanism of ABA signaling.
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The ProteomeXchange consortium in 2017: supporting the cultural change in proteomics public data deposition
Eric W. Deutsch,Attila Csordas,Zhi Sun,Andrew F. Jarnuczak,Yasset Perez-Riverol,Tobias Ternent,David S. Campbell,Manuel Bernal-Llinares,Shujiro Okuda,Shin Kawano,Robert L. Moritz,Jeremy Carver,Mingxun Wang,Mingxun Wang,Yasushi Ishihama,Nuno Bandeira,Nuno Bandeira,Henning Hermjakob,Henning Hermjakob,Juan Antonio Vizcaíno +19 more
TL;DR: The ProteomeXchange Consortium of proteomics resources was formally started in 2011 to standardize data submission and dissemination of mass spectrometry proteomics data worldwide and is supporting a change in culture of the proteomics field.