Patent
Non-isotopic in-situ hybridization method for detection of nucleic acids
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TLDR
In this article, improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer major increases of resolution, sensitivity, and simplicity unavailable in previously known techniques.Abstract:
Improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer major increases of resolution, sensitivity, and simplicity unavailable in previously known techniques. The methodology is able to detect even a single-copy of a specific nucleic acid of interest under controlled conditions regardless of whether these are DNA or RNA sequences; or whether the nucleic acid sequence of interest is localized in the chromosomes, nucleus, or cytoplasm of a cell. The methods employ a variety of non-isotopic labels and detection means for rapid and reliable assays. The invention is also provided in kit form for use in the clinical/diagnostic laboratory such that a relatively unskilled person can accurately and reproducibly detect even a single-copy of a specific nucleic acid of interest.read more
Citations
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PatentDOI
IMAGING INDIVIDUAL mRNA MOLECULES USING MULTIPLE SINGLY LABELED PROBES
Arjun Raj,Sanjay Tyagi +1 more
TL;DR: In this article, a method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, including at least 30 non- overlapping probe binding regions of 15-100 nucleotides, was proposed.
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Image processing and analysis of individual nucleic acid molecules
TL;DR: In this article, a method for observing and determining the size of individual molecules and for determining the weight distribution of a sample containing molecules of varying size is presented. But the method is not suitable for the measurement of large molecules, such as nucleic acids.
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Extended depth of field imaging for high speed object analysis
William E. Ortyn,David Basiji,Keith Frost,Luchuan Liang,Richard Bauer,Brian E. Hall,David J. Perry +6 more
TL;DR: In this article, an optical distortion element is introduced into the flow imaging system, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field.
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Blood and cell analysis using an imaging flow cytometer
William E. Ortyn,David Basiji,Philip J. Morrissey,Thaddeus C. George,Brian E. Hall,Cathleen A. Zimmerman,David J. Perry +6 more
TL;DR: In this article, multimodal/multispectral images of a population of cells are simultaneously collected and morphometric and/or morphometric features identifiable in the images are used to separate the population into a plurality of subpopulations.
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Detection of circulating tumor cells using imaging flow cytometry
TL;DR: In this paper, the authors use morphometric and morphometric features derived from multi-mode images of cells in flow to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged.
References
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Journal ArticleDOI
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
TL;DR: The use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations is described and chromosomes in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase spreads and interphase nuclei when human genomic DNA was used as a probe.
Journal ArticleDOI
Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.
Mary E. Harper,Grady F. Saunders +1 more
TL;DR: In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling of band p36 of chromosome 1, such that 22% of all chromosome grains were located on this region.
Journal ArticleDOI
Immunological method for mapping genes on Drosophila polytene chromosomes
TL;DR: This immunological approach offers four advantages over conventional autoradiographic procedures for detecting in situ hybrids: the time required to determine the site of hybridization is decreased markedly, the biotin-labeled probes are chemically stable and give reproducible results for many months, and the resolving power is equal to and often greater than that achievedautoradiographically.
Journal ArticleDOI
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes
David J. Brigati,David Myerson,David Myerson,Jeffry J. Leary,Barbara Spalholz,Susan Z. Travis,Caroline K.Y. Fong,Caroline K.Y. Fong,G. D. Hsiung,G. D. Hsiung,David C. Ward +10 more
TL;DR: The visualization of parvov virus, polyomavirus, herpes simplex virus, adenovirus, and retrovirus genetic material in infected cell cultures and herpessimplex and adenvirus DNA in paraffin-embedded autopsy tissues is reported.
Patent
Modified nucleotides and methods of preparing and using same
TL;DR: In this paper, the structure of polynucleotide sequences in chromosomes, fixed cells, tissue sections, and cell extracts has been studied for detection and localization of DNA-RNA sequences.