Erik Gentalen

TL;DR: A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome), finding that 17 percent were essential for viability in rich medium.

TL;DR: In this article, a method based on photolithography and surface fluorescence was used to determine photodeprotection rates and stepwise yields for light-directed oligonucleotide synthesis using photolabile 5')-(((α-methyl-2-nitropiperonyl)oxy)carbonyl)(MeNPOC)-2'deoxynucleoside phosphoramidites on planar glass substrates.

TL;DR: This digestion demonstrated that the dsDNA arrays can be further biochemically modified and that the DNA is accessible for interaction with DNA-binding proteins, and could be extended to explore the spectrum of sequence-specific protein binding sites in genomes.

TL;DR: A previously undescribed isoelectric focusing technology allows cell signaling to be quantitatively assessed in <25 cells by analyzing pan-specific antibody signals from individual resolved forms of a protein, without the problems associated with using multiple antibodies with different binding avidities to detect individual protein forms.

TL;DR: In this article, the authors provide arrays of polynucleotide probes having at least one pooled position, which can be used for linkage analysis, sequence analysis, and expression monitoring, and provide methods of using such arrays for e.g., linkage analysis.