Journal ArticleDOI
Non-linear accumulation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidized DNA damage, during aging.
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TLDR
The results indicate that the accumulation of 8-OHdG in the DNA of rat organs begins at ages above 24 months, and this biomarker is a proposed biomarker for DNA damaged by oxidative stress.Abstract:
Damage to DNA seems to be involved in aging and the etiology of age-associated degenerative diseases. The purpose of this study is to examine changes in DNA damage during aging. An oxidized nucleoside, 8-hydroxy-2'-deoxyguanosine (8-OHdG), is a proposed biomarker for DNA damaged by oxidative stress. The content of 8-OHdG in nuclear DNA isolated from brain, heart, liver, and kidneys of male Fischer 344 rats of different ages was measured, 8-OHdG can be detected selectively and sensitively at the fmol level by high performance liquid chromatography-electrochemical detection at an applied potential of +350 mV. The amount of 8-OHdG, expressed as the ratio to deoxyguanosine in nuclear DNA, in heart, liver, and kidney remained steady from 2 to 24 months and then increased progressively. The content of 8-OHdG in the DNA in brain showed no changes from 2 to 27 months, but was significantly higher in 30 month-old rats. There was a significant 2-fold increase in the amount of 8-OHdG in the nuclear DNA of all organs tested in 30 month-old rats as compared to 2-24 month-old rats. These results indicate that the accumulation of 8-OHdG in the DNA of rat organs begins at ages above 24 months.read more
Citations
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Journal ArticleDOI
The Free Radical Theory of Aging Matures
Kenneth B. Beckman,Bruce N. Ames +1 more
TL;DR: The status of the free radical theory of aging is reviewed, by categorizing the literature in terms of the various types of experiments that have been performed, which include phenomenological measurements of age-associated oxidative stress, interspecies comparisons, dietary restriction, and the ongoing elucidation of the role of active oxygen in biology.
Journal ArticleDOI
Oxidative Decay of DNA
Kenneth B. Beckman,Bruce N. Ames +1 more
TL;DR: A proliferation of techniques has resulted in the confirmation of the early hypotheses and also delivered some surprises in the study of DNA oxidation, and some of the most interesting recent results are outlined.
Journal ArticleDOI
Does oxidative damage to DNA increase with age
Michelle L. Hamilton,H. Van Remmen,J. A. Drake,Hong Yang,Zhongmao Guo,K. Kewitt,Christi A. Walter,Arlan Richardson +7 more
TL;DR: Dietary restriction was shown to significantly reduce the age-related accumulation of oxo8dG levels in nDNA in all tissues of male B6D23F1 mice and in most tissues ofmale F344 rats, and it was shown that dietary restriction prevented theAge-related increase in oxo 8dG Levels in mtDNA isolated from the livers of both rats and mice.
Journal ArticleDOI
DNA oxidation matters: The HPLC–electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine
Harold J. Helbock,Kenneth B. Beckman,Mark K. Shigenaga,Patrick B. Walter,Alan A. Woodall,Helen C. Yeo,Helen C. Yeo,Bruce N. Ames +7 more
TL;DR: In this paper, the authors examined difficulties inherent in the analysis of oxo8dG, identified sources of artifacts, and provided solutions to some of the common methodological problems, concluding that phenol extraction of DNA contributes a real but minor increase in the measured level of oxoxo8DG when compared, under equivalent conditions, with a successful nonphenol method.
DNA oxidation matters: The HPLC- electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine (adductsyagingycancerymutationyoxidants)
Harold J. Helbock,Kenneth B. Beckman,M Ark K. Shigenaga,Atrick B. Walter,A Lan A. Woodall,Helen C. Yeo,Bruce N. Ames +6 more
TL;DR: It is found that phenol extraction of DNA contributes a real but minor increase in the level of oxo8dG when compared, under equivalent conditions, with a successful nonphenol method, and a more significant reduction in the baseline level was achieved with a modification of the recently introduced chaotropic NaI method.
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