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Journal ArticleDOI

Online measurement of dissolved oxygen in shake flask to elucidate its role on caffeine degradation by Pseudomonas sp.

TLDR
Higher DO in the medium is required for efficient conversion of caffeine to theobromine which is in agreement that the reaction is oxidative demethylation which will be useful in shaking flask studies and scaling up processes.
Abstract
Caffeine is a plant alkaloid present in the large ratio over other emerging pollutants and it causes serious health effects on overdosage. Microbial degradation of caffeine produces metabolites tha...

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Citations
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Self-directing optimization for enhanced caffeine degradation in synthetic coffee wastewater using induced cells of Pseudomonas sp.: Bioreactor studies

TL;DR: In this paper, a regression model was developed to predict the caffeine degradation rate with oxygen mass transfer coefficient (kLa) and initial biomass concentration, and the optimal conditions of initial biomass and kLa were 0.23g/L and 64.26h−1 respectively.
Journal ArticleDOI

Monitoring of Oxygen, pH, CO 2 , and Biomass in Smart Single‐Use Shake Flasks

TL;DR: In this article , optical sensors are employed in single-use shake flasks to measure oxygen, carbon dioxide, pH, and biomass, and optical sensors can also be used to measure carbon dioxide and carbon dioxide.
Journal ArticleDOI

Optimization by uniform design U8(83) approach for enhanced caffeine degradation in synthetic wastewater in bioreactor

TL;DR: This is the first‐ever bioreactor study showing highest caffeine degradation rate in synthetic coffee wastewater with limited experimental runs.
References
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Journal ArticleDOI

Mechanisms for the reduction of caffeine consumption: What, how and why.

TL;DR: Consumers use a wide range of techniques when attempting to reduce caffeine consumption, but the current study found consumers most frequently focus on substance and behavioural substitution.