Journal ArticleDOI
Patterns of wall synthesis inSaccharomyces cerevisiae
Reads0
Chats0
TLDR
Wall formation inSaccharomyces cerevisiae seems to be the result of two main patterns of wall material deposition: around the whole periphery of the cell in nonbudding ones and mainly at the tip of the daughter cell or at the cross wall that separates dividing cells.Abstract:
Wall formation inSaccharomyces cerevisiae seems to be the result of two main patterns of wall material deposition: (i) around the whole periphery of the cell in nonbudding ones, and (ii) mainly at the tip of the daughter cell or at the cross wall that separates dividing cells. This interpretation has been obtained following experiments in which RNA or protein synthesis has been inhibited. Under these conditions, glucan formation takes place, and wall thickening is probably due to the accumulation of this polysaccharide. Furthermore, once a pattern of wall deposition has been established, it is not modified by inhibition of RNA or protein synthesis.read more
Citations
More filters
Journal ArticleDOI
Tunicamycin and papulacandin B inhibit incorporation of specific mannoproteins into the wall of Candida albicans regenerating protoplasts.
TL;DR: Partially regenerated walls were able to incorporate 'in vitro' non-covalently bound mannoproteins, indicating that some components of very large cellular structures such as walls are capable of being articulated by a self-assembly process.
Book ChapterDOI
Biogenesis of the Fungal Cell Wall
TL;DR: There are many data supporting the idea that temporal and spatial regulation of wall polymer synthesis and assembly are critical for the properties of the walls, which thus do not exclusively depend on their chemical composition, but also on the way that different polymers interact.
Journal ArticleDOI
Metabolism of Saccharomyces cerevisiae envelope mannoproteins
TL;DR: The results suggest that at least part of the mannoproteins initially found in the periplasmic space may move into the wall, and the time lag between the addition of the radioactive precursors and their incorporation in the cell envelope indicates that protein formation and carbohydrate incorporation take place in succession.
Journal ArticleDOI
The glucan-chitin complex in Saccharomyces cerevisiae
TL;DR: Scar rings (SR) from scarless cells at the early stages of budding and mature bud scars from Saccharomyces cerevisiae isolated by both chemical and enzymic treatment of cell walls were observed by selected-area electron diffraction (SAED), X-ray diffraction and electron microscopy with simultaneous physico-chemical characterization of α-chitin and glucan.
Book ChapterDOI
Biogenesis of the yeast cell wall.
TL;DR: It seems clear that the walls of yeast cells must be structurally and enzymatically a complex organelle, increased by the fact that different proteins with important roles in the cell economy as well as survival products are localized on the wall or in the periplasmic space.
References
More filters
Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
Journal ArticleDOI
Chitin synthetase zymogen is attached to the yeast plasma membrane.
TL;DR: The results confirm the previous hypothesis that the chitin synthetase zymogen is associated with the plasma membrane, a basic assumption for the explanation of localized activation of the enzyme and initiation of septum formation.
Journal ArticleDOI
Temperature-sensitive Yeast Mutant Defective in Ribonucleic Acid Production
H. T. Hutchison,H. T. Hutchison,Leland H. Hartwell,Calvin S. McLaughlin,Calvin S. McLaughlin +4 more
TL;DR: It is concluded that mutant ts(-) 136 is defective either in the synthesis of all types of cy toplasmic RNA, or in the transport of newly synthesized RNA from the nucleus to the cytoplasm, and that the mRNA of a eucaryotic organism (yeast) is metabolically unstable, having a half-life of approximately 23 min at 36 C.