Journal ArticleDOI
Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA.
TLDR
The binding capacity has been found to be significantly higher for sc plasmid, probably because of its compact structure, being also improved when using feedstock with increased plasmids concentrations and decreased linear velocity.About:
This article is published in Journal of Chromatography A.The article was published on 2011-04-01. It has received 40 citations till now.read more
Citations
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Journal ArticleDOI
Current trends in separation of plasmid DNA vaccines: A review
TL;DR: This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications and special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery.
Journal ArticleDOI
Advances in chromatographic supports for pharmaceutical‐grade plasmid DNA purification
TL;DR: The present review discusses the structural progress and evolution of the chromatographic supports that have been used for plasmid DNA purification, and focuses on the chemical and structural classification of the different media and on some of the specific ligands used forplasmidDNA bioseparation.
Journal ArticleDOI
Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications.
TL;DR: The analytical results and transfection studies performed with the pDNA sample purified with this monolithic enabling technology, confirmed the suitability of this pDNA to be used in pharmaceutical applications.
Journal ArticleDOI
Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support.
TL;DR: A method that combines the high selectivity of arginine affinity ligands with the versatility of monoliths to efficiently purify the supercoiled plasmid HPV-16 E6/E7 can be the key to obtain an adequate non-viral vaccine against a HPV infection.
Journal ArticleDOI
Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification.
TL;DR: A new enabling technology is presented to obtain the completely purified non-viral vector, able to act with good efficiency as gene therapy delivery vehicle in several diseases like cancer.
References
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Journal ArticleDOI
Amino acid–base interactions: a three-dimensional analysis of protein–DNA interactions at an atomic level
TL;DR: The majority of amino acid-base interactions observed follow general principles that apply across all protein-DNA complexes, although there are individual exceptions.
Journal ArticleDOI
Affinity monolith chromatography.
Rangan Mallik,David S. Hage +1 more
TL;DR: This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes.
Journal ArticleDOI
Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion.
TL;DR: Examples of applications for protein and polynucleotide separation performed on monoliths are given and enzymatic conversion was described showing the examples of several immobilzed enzymes.
Journal ArticleDOI
Plasmid DNA purification.
TL;DR: The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non‐viral over viral vectors.
Journal ArticleDOI
Chromatography of plasmid DNA.
TL;DR: The specificity of pDNA purification and the consequent limitations to the performance of chromatography are described.