Journal ArticleDOI
Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions of Lycopersicon esculentum.
TLDR
RAPDs appear promising for both germplasm fingerprinting and as a predictor of genetic diversity for plant breeding applications.Abstract:
Random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers were used to assess the variability in tomato germplasm (Lycopersicon esculentum Mill.), which in...read more
Citations
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Book ChapterDOI
Contributions of PCR-Based Methods to Plant Systematics and Evolutionary Biology
Andrea D. Wolfe,Aaron Liston +1 more
TL;DR: There are many other techniques aside from DNA sequencing in which PCR is appropriate and extremely useful, and these applications of PCR in plant systematics are reviewed.
Journal ArticleDOI
Genome Mapping and Molecular Breeding of Tomato
TL;DR: The cultivated tomato, Lycopersicon esculentum, is the second most consumed vegetable worldwide and a well-studied crop species in terms of genetics, genomics, and breeding.
Book ChapterDOI
DNA Markers and Plant Breeding Programs
TL;DR: This chapter attempts to survey the status of selected applications of DNA fingerprinting for activities of common interest to plant breeding programs and, ultimately, crop improvement.
Journal ArticleDOI
Distribution of SUN, OVATE, LC and FAS in the Tomato Germplasm and the Relationship to Fruit Shape Diversity
Gustavo Rubén Rodríguez,Stéphane Muños,Claire Anderson,Sung-Chur Sim,Andrew P. Michel,Mathilde Causse,Brian B. McSpadden Gardener,David M. Francis,Esther van der Knaap +8 more
TL;DR: The data suggested that the LC, FAS, and SUN mutations arose in the same ancestral population while the OVATE mutation arose in a separate lineage, whereas the SUN mutation appeared to be a postdomestication event arising in Europe.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Book
Molecular Evolutionary Genetics
TL;DR: Recent developments of statistical methods in molecular phylogenetics are reviewed and it is shown that the mathematical foundations of these methods are not well established, but computer simulations and empirical data indicate that currently used methods produce reasonably good phylogenetic trees when a sufficiently large number of nucleotides or amino acids are used.
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Related Papers (5)
RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon.
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Mathematical model for studying genetic variation in terms of restriction endonucleases
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