Journal ArticleDOI
Phosphorylation-dephosphorylation of pyruvate dehydrogenase complex from pea leaf mitochondria.
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TLDR
Evidence is taken as conclusive evidence that the pyruvate dehydrogenase complex in pea leaf mitochondria undergoes interconversion between deactivated and activated states by covalent modification (phosphorylation-dephosphorylated) catalyzed by a kinase and phosphatase.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1981-04-01. It has received 49 citations till now. The article focuses on the topics: Mitochondrial pyruvate dehydrogenase complex & Pyruvate decarboxylation.read more
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Book ChapterDOI
Biosynthesis of Starch and Its Regulation
TL;DR: The first in vitro demonstration of (1→4)-α-glucosidic linkage formation in plants occurred in 1940 when Hanes (1940) demonstrated the formation of an amylose product from glucose-1-P using potato extracts [reaction (1)].
Journal ArticleDOI
Protein phosphorylation as a mechanism for regulation of spinach leaf sucrose-phosphate synthase activity.
TL;DR: In the in vitro system, the incorporation of radiolabel into the 120-kDa subunit of SPS was dependent on time and magnesium concentration, and was closely paralleled by inactivation of the enzyme.
Journal ArticleDOI
Purification and Characterization of the Pea Chloroplast Pyruvate Dehydrogenase Complex A Source of Acetyl-CoA and NADH for Fatty Acid Biosynthesis
TL;DR: Characterization of the substrates as pyruvate, NAD(+), and coenzyme-A and the products as NADH, CO(2), and acetyl-CoA, in a 1:1:1 stoichiometry unequivocally established that activity was the result of the pyruve dehydrogenase complex.
Journal ArticleDOI
Light Regulation of Leaf Mitochondrial Pyruvate Dehydrogenase Complex' Role of Photorespiratory Carbon Metabolism
Joanna Gemel,Douglas D. Randall +1 more
TL;DR: It is concluded that the photorespiratory glycine to serine conversion that occurs in C(3) leaf mitochondria can provide the NADH to drive oxidative phosphorylation and subsequent inactivation of mtPDC.
References
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Journal ArticleDOI
Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System
TL;DR: The retardation coefficient is shown both empirically and theoretically to be a uniform function of molecular weight of protein-SDS complexes over specified ranges, providing a rationale for determining molecular weight from plots of the negative logarithm of relative mobility against molecular weight.
Journal ArticleDOI
Phosphorylation of chloroplast membrane polypeptides
TL;DR: Protein phosphorylation is a reversible, energy-dependent membrane modification, but it differs from the other changes in that it takes the form of a specific chemical reaction involving certain identifiable chloroplast membrane polypeptides.
Journal ArticleDOI
Regulation of mammalian pyruvate dehydrogenase.
Richard M. Denton,P. J. Randle,Barbara J. Bridges,Ronald H. Cooper,Alan L. Kerbey,H T Pask,David L. Severson,David Stansbie,Susan Whitehouse +8 more
TL;DR: It is presented that in both adipose tissue and heart muscle, differences in activity of pyruvate dehydrogenase in the intact tissue persist during preparation and subsequent incubation of mitochondria.
Journal ArticleDOI
Chloroplast Phosphoproteins. Evidence for a Thylakoid‐Bound Phosphoprotein Phosphatase
TL;DR: The enzyme responsible for dephosphorylation is a phosphatase rather than a phosphotransferase or the thylakoid protein kinase acting in reverse and does not require phosphoryl group acceptors such as ADP.
Journal ArticleDOI
Chloroplast phosphoproteins. The protein kinase of thylakoid membranes is light-dependent
TL;DR: It is shown that the protein kinase responsible for the $o:;phorylation is also bound to the thylakoids and is itself light-dependent, Protein kinases generaily utilize [y-32P]ATP as phosphoryl group donor in in vitro assays.