Patent
Pichia pastoris gene sequences and methods for their use
TLDR
In this article, regulatory nucleotide sequences for a novel Pichia pastoris gene, designated PpSEC10 gene, and respective amino acid sequences for the secretion leader and mature Sec10p protein components of the precursor polypeptide encoded by this novel gene are provided.Abstract:
Regulatory nucleotide sequences for a novel Pichia pastoris gene, designated PpSEC10 gene, and the nucleotide sequences and respective amino acid sequences for the secretion leader and the mature Sec10p protein components of the precursor polypeptide encoded by this novel gene are provided. These compositions are useful in methods for expression and secretion of proteins when assembled in proper reading frame, individually or in combination, within a DNA construct that further comprises a nucleotide sequence encoding a protein of interest. Vectors comprising the DNA constructs of the invention can be used to transform a yeast host cell, which can then be cultured to obtain the secreted protein of interest. Kits useful in this method and in methods of detection of the Sec10p protein using antibodies are also disclosed.read more
Citations
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References
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Journal ArticleDOI
Foreign gene expression in yeast: a review.
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Journal ArticleDOI
Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris
Koti Sreekrishna,Robert G. Brankamp,Keith E. Kropp,Dale T. Blankenship,Jiu-Tsair Tsay,Phil L. Smith,Jonathan D. Wierschke,A Subramaniam,Lori A. Birkenberger +8 more
TL;DR: The inherent ability of P. pastoris to convert the zymogen (pro-enzyme) form of matrix metalloproteinases (MMP) into active mature forms (which tend to self-degrade, and in some instances also cause damage to cells), largely limits the use of this system for the production of MMP, but this problem can be partly alleviated by co-expression of tissue inhibitor of M MP (TIMP-1).
Patent
Production of insulin-like growth factor-1 in methylotrophic yeast cells
Russell Arthur Brierley,Geneva Ruth Davis,Gregory Clyde Holtz,Martin A. Gleeson,Bradley Drake Howard +4 more
TL;DR: In this article, the authors used recombinant methylotrophic yeast strains for the expression and secretion of insulin-like growth factor-1 (IGF-1), a naturally occurring, relatively short, single chain polypeptide.
Patent
Improved expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
TL;DR: In this article, a yeast α-factor expression system is provided comprised of a truncated leader sequence, containing the α factor signal peptide and one glycosylation site, linked by a processing site to a non-yeast protein-encoding sequence.
Patent
Genes which influence pichia proteolytic activity, and uses therefor
TL;DR: In this article, the isolation and characterization of genes involved in proteolytic processing in species of the genus Pichia is described, which has enabled the generation of strains of Pichias which are deficient in proteocyte activity, which is useful as hosts for the expression of proteolytically sensitive recombinant products.