Jeffrey J. Clare

TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.

TL;DR: In this paper, the tetanus toxin fragment C was induced to 27% of total cell protein or about 12 g/l of culture in high biomass fermentations and the level of expression was largely independent of the site of chromosomal integration of the gene (AOX1 or HIS4), the type of integrant (insertion or transplacement), and the methanol utilisation phenotype of the host strain (Mut+ or Muts).

TL;DR: To increase the level of secreted mEGF, a synthetic secretion cassette encoding the alpha-factor prepro leader peptide from Saccharomyces cerevisiae fused to mouse epidermal growth factor was constructed and a method for rapidly screening large numbers of P. pastoris transformants for the presence of many copies of a foreign gene was developed.

TL;DR: In this article, the use of vectors containing the Tn903 kanr gene conferring G418-resistance was reported, which showed that copy number showed a tight correlation with drug resistance.

TL;DR: It is shown that retigabine acts as a KCNQ potassium channel opener, and it is likely that M-current modulation can explain the anticonvulsant actions of retIGabine in animal models of epilepsy.