Journal ArticleDOI
Foreign gene expression in yeast: a review.
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TLDR
In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.Abstract:
0749-503X/92/060423-66 $38.00read more
Citations
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Microbial cellulose utilization: fundamentals and biotechnology.
TL;DR: A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts.
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Heterologous protein expression in the methylotrophic yeast Pichia pastoris
TL;DR: This paper reviews the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced and describes new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
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Recent advances in the expression of foreign genes in Pichia pastoris
TL;DR: The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest and improvements in understanding and application have improved its utility even further.
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Glucose oxidase--an overview.
TL;DR: Various purification techniques for higher recovery of glucose oxidase are described here, and issues of enzyme kinetics, stability studies and characterization are addressed.
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Recombinant protein expression in Pichia pastoris.
TL;DR: Major advances in the development of new strains and vectors, improved techniques, and the commercial availability of these tools coupled with a better understanding of the biology of Pichia species have led to this microbe’s value and power in commercial and research labs alike.
References
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Journal ArticleDOI
Transformation of intact yeast cells treated with alkali cations.
TL;DR: The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Journal ArticleDOI
Assembly of asparagine-linked oligosaccharides.
TL;DR: The structure of ASPARAGINE-LINKed OLIGOSACCI-IARIDES and transfer-Oligosaccharide Structural Requirements, and Sequence of Processing and Specificity of Processing Enzymes are presented.
Journal ArticleDOI
Protein folding in the cell.
TL;DR: Folding and assembly of polypeptides in vivo involves other proteins, many of which belong to families that have been highly conserved during evolution.
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A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation
Gray D. Shaw,Robert Kamen +1 more
TL;DR: It is proposed that the AU sequences are the recognition signal for an mRNA processing pathway which specifically degrades the mRNAs for certain lymphokines, cytokines, and proto-oncogenes.
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The scanning model for translation: an update.
TL;DR: The small (40S) subunit of eukaryotic ribosomes is believed to bind initially at the capped 5'-end of messenger RNA and then migrate, stopping at the first AUG codon in a favorable context for initiating translation.