Journal ArticleDOI
Preparation of 2-acetamido-2-deoxy-β-d-glucose oligosaccharides from acid hydrolyzates of chitin by electrolytic desalting and exclusion chromatography
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This article is published in Analytical Biochemistry.The article was published on 1972-04-01. It has received 10 citations till now. The article focuses on the topics: Chitin.read more
Citations
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Journal ArticleDOI
An exo-β-N-acetylglucosaminidase from Bacillus subtilis B; characterization
TL;DR: It is concluded that the highly purified exo-β-N-acetylglucosaminidase is entirely specific for substrates with non-reducing N-acetamido-2-deoxy-d-glucopyranosyl end groups.
Journal ArticleDOI
A glycosynthase derived from an inverting GH19 chitinase from the moss Bryum coronatum.
TL;DR: The S102A mutant showed the greatest glycosynthase activity owing to its enhanced F(-) releasing activity and its suppressed hydrolytic activity, and this is the first report on a glycoslynthase that employs amino sugar fluoride as a donor substrate.
Journal ArticleDOI
Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from Arabidopsis thaliana, AtChiC
TL;DR: The transglycosylation reaction to be significantly enhanced in G74W-NtChiV and G75W-AtChiC when compared with the corresponding wild-type enzymes, suggesting that the introduced tryptophan side chain might protect the oxazolinium ion intermediate from attack by a nucleophile water molecule.
Journal ArticleDOI
Characterization of an Acidic Chitinase from Seeds of Black Soybean (Glycine max (L) Merr Tainan No. 3)
TL;DR: Results suggested that this enzyme was an endo-splitting chitinase with short substrate cleavage activity and useful for biotechnological applications, in particular for the production of N-acetyl chitooligosaccharides.
Patent
High yield preparation of dimeric to decameric chitin oligomers
TL;DR: In this article, a new process for the production of chitin oligomers, including fragmentation, partial hydrolysis and recovery, was proposed, which produces high yields, especially of higher (tetra-to deca-) chitIN oligomers.
References
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Journal ArticleDOI
The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular-weight substrates for lysozyme.
TL;DR: Experiments studying the lysozyme-catalyzed hydrolysis of these oligosaccharides showed a large effect of chain length upon the rate of Hydrolysis, and no effect of ionic strength.
Journal ArticleDOI
The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides
TL;DR: A method is described for the separation and estimation of a large number of biologically important monosaccharides including hexuronic acids and certain N-acetylglucosamine derivatives such as muramic acid and N -acetyl glucosaminylasparagine.
Journal ArticleDOI
Separation of glycosaminoglycan saccharide and glycoside mixtures by gel filtration.
Michael A. Raftery,T. Rand-Meir,Frederick W. Dahlquist,S. M. Parsons,C.L. Borders,R. G. Wolcott,W. Beranek,L. Jao +7 more
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Separation of chitin oligosaccharides by thin-layer chromatography.
R.F. Powning,H. Irzykiewicz +1 more
TL;DR: A linear relationship has been established between RM value and degree of polymerisation of oligosaccharide for all solvents used and Ammonia addition to Solvents decreased RF values and increased resolution.