Patent
Process for detecting polymorphisms on the basis of nucleotide differences
TLDR
In this paper, a process for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on nucleic acids and comparing the extension reaction products is described.Abstract:
A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.read more
Citations
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TL;DR: The arbitrarily primed polymerase chain reaction (AP-PCR) method as discussed by the authors is suitable for the identification of bacterial species and strains, including Staphylococcus and Streptococcus species, mammals and plants.
References
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Patent
Process for amplifying nucleic acid sequences
TL;DR: In this article, a process for amplifying any desired specific nucleic acid sequence contained in a mixture of nucleic acids or mixture thereof is described, which can be repeated as often as desired.
Patent
Process for amplifying, detecting, and/or-cloning nucleic acid sequences
Kary Banks Mullis,Norman Arnheim,Randall K. Saiki,Henry A. Erlich,Glenn Thomas Horn,Stephen J. Scharf +5 more
TL;DR: In this paper, the authors proposed a method for synthesizing nucleic acid sequences using primers, which can be repeated stepwise or simultaneously and can be replicated as often as desired.
Journal ArticleDOI
Simultaneous analysis of multiple polymorphic loci using amplified sequence polymorphisms (ASPs).
Mark H. Skolnick,R B Wallace +1 more
TL;DR: A systematic approach to gene mapping and genotyping based on the simultaneous analysis of multiple amplified sequence polymorphisms (ASPs), which measure variation in DNA sequences which have been amplified by a polymerase and/or a ligase is presented.
Patent
Amplification of nucleic acid sequences using random sequence oligonucleotides as primers
TL;DR: In this paper, a process for substantially amplifying template nucleic acid present in a sample is described, wherein the amplification may be performed without the need for prior knowledge of specific sequences.