Journal ArticleDOI
Production of haploid plantlets in anther cultures of Albizzia lebbeck L.
TLDR
Anthers of Albizzia lebbeck on B5 medium supplemented with kinetin and 2, 4-D showed callus initiation from microspores, confirming the microspore origin of the regenerants.Abstract:
Anthers of Albizzia lebbeck on B5 medium (BM) supplemented with kinetin (2 mg/l) and 2, 4-D (0.5 mg/l) showed callus initiation from microspores. Differentiation of embryoids and shoots was obtained on BM + BAP (1 mg/l) + IAA (0.5 mg/l) and of roots on BM. Root tip squashes of the regenerated plantlets showed the haploid chromosome number (n=13), confirming the microspore origin of the regenerants.read more
Citations
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Journal ArticleDOI
Cytogenetics of plant cell and tissue cultures and their regenerates
Francesco D'Amato,M. W. Bayliss +1 more
TL;DR: The cytogenetics of plant cell and tissue cultures and their regenerates will be discussed, and nuclear processes at and during callus induction will be followed.
Book ChapterDOI
Application of micropropagation to forestry
TL;DR: There is an urgent need for larger numbers of improved, fast-growing trees, and the tree improvement programs underway and the clonal propagation methods available offer only limited possibilities of achieving this goal.
Book ChapterDOI
Published doubled haploid protocols in plant species
TL;DR: These protocols provide procedures for production of DH in such major crops as wheat, maize, barley, rapeseed or potato and also in other crop species where this technology is more advanced, or in other words, where a relatively high frequency of doubled haploids was obtained.
Journal ArticleDOI
Toward doubled haploid production in the Fabaceae: Progress, constraints, and opportunities
Janine Croser,Monika M. Lulsdorf,P.A. Davies,Heather Clarke,K.L. Bayliss,Nalini Mallikarjuna,Kadambot H. M. Siddique +6 more
TL;DR: A review of the literature has enabled us to identify some general similarities between the protocols developed for haploid plant induction across the various legumes, which will assist researchers to identify approaches suited to their target Fabaceae species.
References
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Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
BookDOI
Applied and fundamental aspects of plant cell, tissue, and organ culture.
TL;DR: This book comprises 34 articles on regeneration of plants, vegetative propagation and cloning; haploids; cytology, cytogenetics and plant breeding; protoplasts, somatic hybridization and genetic engineering; plant pathology; secondary products and a chapter on isoenzymes, radiobiology, and cryobiology of plant cells.
Journal ArticleDOI
Cell Division and Differentiation of Embryos in the Pollen Grains of Datura in vitro
Sipra Guha,Satish C. Maheshwari +1 more
TL;DR: Evidence is reported which shows that the mature anthers of Datura produce numerous embryo-like structures when cultured on Nitsch's medium containing 15 per cent coconut milk originate from the pollen grains.
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
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