Journal ArticleDOI
Protein partitioning in two-phase aqueous polymer systems.
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TLDR
Theories of protein partitioning in two-phase polymer systems which account for the effects of different aspects of system composition are reviewed in this article, where some recommendations for future work are given.Abstract:
Theories of protein partitioning in two-phase polymer systems which account for the effects of different aspects of system composition—such as the choice of materials, protein size, polymer molecular weight, polymer concentration, salt concentration, and affinity ligands—are reviewed. Although the present models provide some information about specific aspects of partitioning, a comprehensive and fundamental theory which can be used to predict protein partitioning behavior has not yet been developed. Some recommendations for future work are given.read more
Citations
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Aqueous two-phase systems for protein separation: Studies on phase inversion
TL;DR: In this paper, phase equilibrium studies were performed with the PEG 400phosphate system, obtaining equilibrium binodal lines, tie lines, and phase inversion points, and a method of calculation of the critical point on the binoderal curve is described.
Journal ArticleDOI
Aqueous two-phase systems. A general overview.
TL;DR: Aqueous two-phase systems (ATPS) have proven to be a useful tool for analysis of biomolecular and cellular surfaces and their interactions, fractionation of cell populations, product recovery in biotechnology, and so forth.
Journal ArticleDOI
Iodinated blood pool contrast media for preclinical X-ray imaging applications--a review.
TL;DR: The aim of this review is to propose an overview into the formulation and properties of iodinated micro-CT contrast agents for preclinical applications, i.e. liposomes, nanoemulsions, micelles, dendrimers and other polymeric particles.
Journal ArticleDOI
Liquid–liquid extraction of biomolecules: an overview and update of the main techniques
Priscila Gava Mazzola,André Moreni Lopes,Francislene Andréia Hasmann,Angela Faustino Jozala,Thereza Christina Vessoni Penna,Pérola Oliveira Magalhães,Carlota de Oliveira Rangel-Yagui,Adalberto Pessoa +7 more
TL;DR: An extensive description of each two-phase systems is presented, based on the biomolecules behavior and the tools employed to improve their extraction and purification.
Journal ArticleDOI
Ionic liquid-based aqueous two-phase systems and their applications in green separation processes
TL;DR: In this article, the effects of ionic liquid (IL)-based aqueous two-phase systems (ATPSs) are reviewed and highlighted the possible directions of future developments.
References
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Statistical Theory of the Adsorption of Interacting Chain Molecules. 1. Partition Function, Segment Density Distribution, and Adsorption Isotherms
TL;DR: In this article, a general theory for polymer adsorption using a quasi-crystalline lattice model is presented, where the Bragg-Williams approximation of random mixing within each layer parallel to the surface is adopted.
Journal ArticleDOI
Statistical theory of the adsorption of interacting chain molecules. II. Train, loop, and tail size distribution
J.M.H.M. Scheutjens,G.J. Fleer +1 more
Journal ArticleDOI
Salt effect on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic series.
Wayne Melander,Csaba Horváth +1 more
TL;DR: The analysis of solubility and Chromatographic data shows that the overall salt effect can be described by the two antagonistic effects of salts on electrostatic and hydrophobic interactions.
Journal ArticleDOI
Mechanism of precipitation of proteins by polyethylene glycols. Analysis in terms of excluded volume.
D H Atha,K C Ingham +1 more
TL;DR: Edmond et al. as mentioned in this paper measured the apparent solubilities of various proteins (14,000 to 670,000 daltons) in the presence of polyethylene glycols (PEGs) of different sizes.
Book
Water-Soluble Synthetic Polymers: Volume I: Properties and Behavior
TL;DR: In this article, the physical chemistry of water-soluble synthetic polymers as a group is surveyed and compared, and their properties and behaviour is correlated with their molecular structures for predictive purposes, and sufficient key recent references are also included so that the existing state of the art is delineated.