Purification and properties of a host cell protein required for poliovirus replication in vitro.
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A host cell protein required for poliovirus RNA-dependent RNA replicase activity in vitro has been purified several thousand-fold from an uninfected HeLa cell postmitochondrial supernatant.About:
This article is published in Journal of Biological Chemistry.The article was published on 1982-10-25 and is currently open access. It has received 53 citations till now. The article focuses on the topics: Host factor & RNA-dependent RNA polymerase.read more
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Book ChapterDOI
Replication of Flaviviruses
TL;DR: Flaviviruses were classified as members of the togavirus family until 1984, when the International Committee for the Nomenclature of Viruses voted to make Flaviviridae a separate family.
Journal Article
Functional oligomerization of poliovirus RNA-dependent RNA polymerase.
TL;DR: A model in which poliovirus 3D polymerase functions both as a catalytic polymerase and as a cooperative single-stranded RNA-binding protein during RNA-dependent RNA synthesis is proposed.
Journal ArticleDOI
Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus.
TL;DR: Overall, the kinetics of poliovirus replication (protein synthesis, polymerase activity, and virus production) observed in the HeLa S10-initiation factor in vitro translation reactions were similar to those observed in infected cells.
Journal ArticleDOI
Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.
TL;DR: A cDNA clone encoding the RNA polymerase of poliovirus has been expressed in Escherichia coli under the transcriptional control of a T7 bacteriophage promoter, and the enzyme exhibits poly(A)-dependent oligo(U)-primed poly(U) polymerase activity as well as RNA polymer enzyme activity.
Journal ArticleDOI
Viral RNA polymerases.
Akira Ishihama,Kyosuke Nagata +1 more
TL;DR: This review shows that the viral RNA polymerases are complex in both structure and function, being composed of multiple subunits and carrying multiple functions.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
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Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases
TL;DR: With a Stokes radius measured by the chromatographic method and a sedimentation coefficient determined by density gradient centrifugation, reasonable estimates for both the molecular weight and the frictional ratio (f/f0) of a macromolecule are available.
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Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
James M. Bailey,Norman Davidson +1 more
TL;DR: It appears that complete denaturation of any base-paired secondary structural feature of a nucleic aicd can be achieved at practical concentrations of CH3HgOH.
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