Journal ArticleDOI
Quantitative analysis of free phytosterols by gas chromatography using stationary phase OV-101.
TLDR
An accurate and precise gas chromatographic procedure for the quantitative determination of cholesterol, campesterol, stigmasterol, and β-sitosterol is described and has been applied to the quantitative analysis of tobacco sterols.About:
This article is published in Analytical Biochemistry.The article was published on 1970-03-01. It has received 49 citations till now. The article focuses on the topics: Cholestane & Gas chromatography.read more
Citations
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Journal ArticleDOI
Sterol Distribution in Intracellular Organelles Isolated from Tobacco Leaves
TL;DR: The results support the suggestion that sterols in plant cells may have a physiological function associated with membranes, and the intracellular distribution pattern of cholesterol-(14)C added to the isolation medium provided evidence that the intrACEllular sterol distribution pattern is not an artifact.
Journal ArticleDOI
Sterol Changes during Germination of Nicotiana tabacum Seeds
Parshall B. Bush,C. Grunwald +1 more
TL;DR: During germination, sitosterol was the major sterol in all fractions but stigmasterol and campesterol showed the greatest changes, and the fatty acid composition of the steryl esters and acylated steryl glycosides most closely resembled the di- and triglycerides.
Journal ArticleDOI
Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity
Peter G. Mavrelis,H V Ammon,H V Ammon,John J. Gleysteen,Richard A. Komorowski,Ursula K. Charaf +5 more
TL;DR: The data are consistent with the hypothesis that accumulation of free fatty acids in patients with alcoholic liver disease may be responsible for or contribute to the observed functional and morphological damages.
Journal ArticleDOI
Induction of Fusarium solani mutants insensitive to tomatine, their pathogenicity and aggressiveness to tomato fruits and pea plants
Geneviève Défago,H. Kern +1 more
TL;DR: It is suggested that tomatine is liberated in the very early stages of infection and contributes effectively to the inhibition of further growth of the wild-type strain of F. solani.
Journal ArticleDOI
Interaction of lucerne saponins with sterols
TL;DR: Although lucerne saponins readily form addition products with a variety of sterols, a preferential affinity to cholesterol and 7-dehydrocholesterol was found when the addition product was formed while cholesterol and one of the other sterols were present simultaneously in the reaction mixture.
References
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Quantitative isolation and gas--liquid chromatographic analysis of total dietary and fecal neutral steroids.
TL;DR: The sensitivity of the method is such that I-g fecal aliquots containing as little as 25 pg of mixed neutral steroids can be analyzed accurately, but the procedure lends itself well to preparative scale work for more definitive study of individual neutral steroids.
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Silylations with Bis(trimethylsilyl)acetamide, a Highly Reactive Silyl Donor
TL;DR: In this paper, the silylation of amides, ureas, and amino acids, as well as hindered phenols, carboxylic acids, and enols, is described.
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Characterization of sterols by gas chromatography-mass spectrometry of the trimethylsilyl ethers.
TL;DR: The utility of combined gas chromatography-mass spectrometry in the analysis and characterization of sterols has been explored and positive identification of any of the sterols examined is allowed, whereas application of either technique alone may give inconclusive results.