Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species.
C. Leonards-Schippers,Werner Gieffers,R. Schäfer-Pregl,Enrique Ritter,S J Knapp,Francesco Salamini,Christiane Gebhardt +6 more
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TLDR
Whereas phenotypically no difference in quantitative resistance response was observed between the two fungal races, QTL mapping identified at least one race specific QT locus, conferring race specific resistance to P. infestans.Abstract:
Phytophthora infestans is the most important fungal pathogen in the cultivated potato (Solanum tuberosum). Dominant, race-specific resistance alleles and quantitative resistance--the latter being more important for potato breeding--are found in the germplasm of cultivated and wild potato species. Quantitative trait loci (QTLs) for resistance to two races of P. infestans have been mapped in an F1 progeny of a cross between non-inbred diploid potato parents with multiple alleles. Interval mapping methods based on highly informative restriction fragment length polymorphism markers revealed 11 chromosome segments on 9 potato chromosomes showing significant contrasts between marker genotypic classes. Whereas phenotypically no difference in quantitative resistance response was observed between the two fungal races, QTL mapping identified at least one race specific QT locus. Two QT regions coincided with two small segments on chromosomes V and XII to which the dominant alleles R1, conferring race specific resistance to P. infestans, Rx1 and Rx2, both inducing extreme resistance to potato virus X, have been allocated in independent mapping experiments. Some minor QTLs were correlated with genetic loci for specific proteins related to pathogenesis, the expression of which is induced after infection with P. infestans.read more
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Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight
Junqi Song,James M. Bradeen,S. Kristine Naess,John A. Raasch,Susan M. Wielgus,Geraldine T. Haberlach,Jia Liu,Hanhui Kuang,Sandra Austin-Phillips,C. Robin Buell,John P. Helgeson,Jiming Jiang +11 more
TL;DR: The cloning of the major resistance gene RB in S. bulbocastanum is reported by using a map-based approach in combination with a long-range (LR)-PCR strategy, demonstrating that LR-PCR is a valuable approach to isolate genes that cannot be maintained in the bacterial artificial chromosome system.
Journal ArticleDOI
A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants
TL;DR: The results indicate that plant resistance genes that are effective against nematodes, fungi andviruses and bacteria may be isolated based on common sequence motifs and PCR methodology.
Journal ArticleDOI
Qtl mapping and quantitative disease resistance in plants
TL;DR: These studies provide insights into the number of quantitative resistance loci involved in complex disease resistance, epistatic and environmental interactions, race-specificity of partial resistance locu, interactions between pathogen biology, plant development and biochemistry, and the relationship between qualitative and quantitative loci.
Journal ArticleDOI
Organization of genes controlling disease resistance in the potato genome
TL;DR: It is proposed that some QTL for resistance have a molecular basis similar to single R genes, and genes having structural similarity with cloned R genes and genes involved in the defense response are proposed.
Journal ArticleDOI
Genetic Markers, Map Construction, and Their Application in Plant Breeding
TL;DR: The purpose of this paper is to describe available marker types and examine factors critical for their use in map construction and MAS, and defines the potential use of genetic maps for MAS.
References
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Mapping mendelian factors underlying quantitative traits using rflp linkage maps
Eric S. Lander,David Botstein +1 more
TL;DR: In this paper, a set of analytical methods that modify and extend the classical theory for mapping such quantitative trait loci (QTLs) are described, and explicit graphs are provided that allow experimental geneticists to estimate, in any particular case, the number of progeny required to map QTLs underlying a quantitative trait.
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Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms
Andrew H. Paterson,Eric S. Lander,Eric S. Lander,John Hewitt,Susan Peterson,Stephen E Lincoln,Steven D. Tanksley +6 more
TL;DR: The first use of a complete RFLP linkage map to resolve quantitative traits into discrete Mendelian factors, in an interspecific back-cross of tomato is reported, broadly applicable to the genetic dissection of quantitative inheritance of physiological, morphological and behavioural traits in any higher plant or animal.
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Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers.
TL;DR: Whenever a QTL for grain yield was detected, the heterozygote had a higher phenotype than the respective homozygote suggesting not only overdominance (or pseudooverdominance) but also that these detected QTLs play a significant role in heterosis.
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Molecular-Marker-Facilitated Investigations of Quantitative-Trait Loci in Maize. I. Numbers, Genomic Distribution and Types of Gene Action
TL;DR: Examination of the effects of marked regions on the expression of pairs of traits suggests that genomic regions vary in the direction and magnitudes of their effects on trait correlations, perhaps providing a means of selecting to dissociate some correlated traits.