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Radiation sensitivity of a mutant of Escherichia coli K-12 associated with DNA replication: Evidence for a new repair function
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It is suggested that rer controls the replication of irradiated DNA and thus regulates the coordination between replication and repair of DNA.Abstract:
The isolation and properties of a new radiation sensitive mutant of Escherichia coli K-12 are described which shows a correlation between radiation sensitivity and replication of irradiated DNA. The mutation, called rer, is located between argB and purD loci. The mutant, when grown in tryptone broth after irradiation, is sensitive to UV and γ-rays and incorporates little or no 3H-thymidine but in minimal glucose-salts medium both the radiation sensitivity and incorporation of 3H-thymidine remain identical to that of the parent strain. Studies with a temperature sensitive double mutant rer dnaC show that 1 hr incubation of irradiated cells at 42° C before their transfer to 30° C results in higher survival as compared to their incubation at 30° C only. It is suggested that rer controls the replication of irradiated DNA and thus regulates the coordination between replication and repair of DNA.read more
Citations
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Functions of the gene products of Escherichia coli.
TL;DR: A scheme to categorize cellular functions is used and the occurrence in the E. coli genome of redundant pairs and groups of genes of identical or closely similar function, as well as variation in the degree of density of genetic information in different parts of the genome are discussed.
Journal ArticleDOI
Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map
TL;DR: This map is an update of the edition 9 map by Berlyn et al. and adds new genes discovered and established since 1996 and eliminates those shown to correspond to other known genes.
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coaA and rts are allelic and located at kilobase 3532 on the Escherichia coli physical map.
Woo-joo Song,Suzanne Jackowski +1 more
TL;DR: It is concluded that rts is an allele of the coaA gene, an essential cofactor in numerous biosynthetic and energy-yielding metabolic pathways and a regulator of several key metabolic reactions.
Journal ArticleDOI
Effects of plasmids on chromosome metabolism in bacteria.
TL;DR: The available evidence indicates that plasmids can confer on the cell additional resources which extend its DNA metabolism potential, thereby promoting evolutionary transformations.
References
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Journal ArticleDOI
The disappearance of thymine dimers from dna: an error-correcting mechanism
TL;DR: The onset of DNA synthesis is associated with thymine dimer removal, which is suggested to be one step in cell recovery in resistant strains of E. coli.
Journal ArticleDOI
Isolation and characterization of recombination-deficient mutants of escherichia coli k12
Alvin J. Clark,Ann Dee Margulies +1 more
Journal ArticleDOI
DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes.
TL;DR: Experiments on the mechanisms of DNA repair in Escherichia coli following exposure to irradiation or to mutagens, including alkylating agents and X-rays are reviewed and the role of DNA Repair in genetic recombination is reviewed.
Journal ArticleDOI
A locus that controls filament formation and sensitivity to radiation in escherichia coli k-12.
TL;DR: The greater radiosensitivity of the lon mutants is not due to the loss of the capacity to reactivate uv- photoproducts in DNA because equal numbers of plaques are found when uv irradiated Tl phage is plated on lon and lon/sup +/ strains.
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