Journal ArticleDOI
Random amplified polymorphic DNA fingerprints of the eight taxa of Trichinella and their comparison with allozyme analysis.
Claudio Bandi,G. La Rosa,M. G. Bardin,Giuseppe Damiani,Sergio Comincini,L. Tasciotti,Edoardo Pozio +6 more
TLDR
It is shown that an analogous 8 taxon structure for this genus results from the random amplified polymorphic DNAs (RAPDs), which confirms the potential of RAPDs as a tool for the detection of cryptic species.Abstract:
Eight taxa have recently been proposed as being encompassed by the genus Trichinella on the basis of allozyme and biological data. In this paper we show that an analogous 8 taxon structure for this genus results from the random amplified polymorphic DNAs (RAPDs). Five 10-mer or 20-mer primers were used under different polymerase chain reaction (PCR) conditions to produce multiband RAPD fingerprints from muscle larvae of 40 isolates of Trichinella spp. The resulting RAPD data were analysed following the numerical taxonomic approach, and the resulting classification was compared to that derived from allozyme data. The agreement found between allozymes and RAPDs, while supporting the polyspecific structure of the genus Trichinella, confirms the potential of RAPDs as a tool for the detection of cryptic species. The selected primers were tested on individual muscle larvae in an attempt to standardize a RAPD assay for the routine identification of the 8 taxa of Trichinella. Only 1 of the 5 primers yielded reproducible fingerprints from the single larvae. Using this primer, the 5 species and the 3 other taxa of the genus Trichinella can be identified in a single assay without the need for massive in vivo parasite production.read more
Citations
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Book ChapterDOI
Systematics and epidemiology of trichinella.
Edoardo Pozio,K. Darwin Murrell +1 more
TL;DR: This review describes the current knowledge on the systematics, ecology and epidemiology of Trichinella and trichinellosis, and the impact of recent research discoveries on the understanding of this zoonosis.
Journal ArticleDOI
A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella
TL;DR: The technique was developed further to distinguish genotypes at the level of single muscle larvae using a nested, multiplex PCR, whereby the entire internal transcribed spacer region as well as the gap region of the expansion segment V of the large subunit ribosomal DNA are amplified concurrently in a first-round PCR using primer sets specific for each region.
Journal ArticleDOI
How Does Trichinella spiralis Make Itself at Home
TL;DR: Dickson Despommier presents a hypothesis to account for the formation of the nurse cell-parasite complex of Trichinella spiralis, in which secreted tyvelosylated proteins of the larva play a central role.
Journal ArticleDOI
New pieces of the Trichinella puzzle.
Edoardo Pozio,Dante S. Zarlenga +1 more
TL;DR: This work has ventured to present an up-to-date assessment of the taxonomy, phylogenetic relationships and epidemiology of the genus Trichinella with additional insights on host species, survival strategies in nature and the shortcomings of the current understanding of the epidemiology.
Journal ArticleDOI
Recent advances on the taxonomy, systematics and epidemiology of Trichinella
Edoardo Pozio,Dante S. Zarlenga +1 more
TL;DR: The review herein consolidates the current understanding of the taxonomy, epidemiology, and phylogeny of the genus Trichinella, and identifies areas where data are lacking and the authors' knowledge requires additional clarification.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal Article
The Detection of Disease Clustering and a Generalized Regression Approach
TL;DR: The technic to be given below for imparting statistical validity to the procedures already in vogue can be viewed as a generalized form of regression with possible useful application to problems arising in quite different contexts.
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Journal ArticleDOI
Applications of random amplified polymorphic DNA (RAPD) in molecular ecology
TL;DR: The Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.