Journal ArticleDOI
RAPD Assessment for Identification of Clonal Identity and Genetic Stability of in vitro Propagated Chestnut Hybrids
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TLDR
Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the clonal identity of four in vitro propagated chestnut rootstock hybrids, and polymorphism was detected between the material propagated in vitro and the donor plants they originated from.Abstract:
Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the clonal identity of four in vitro propagated chestnut rootstock hybrids (Castanea sativa × C. crenata) described as originally isolated from the same mother tree. To confirm genetic stability after in vitro multiplication for more than 4 years, RAPD patterns of in vitro and donor plants were compared. From 40 arbitrary 10-mer primers used to amplify DNA, 21 provided patterns and were chosen for comparisons. Although significant differences were found in growth parameters between in vitro material of the putative clones, RAPD profiling showed polymorphism in none but one. This accession may then be withdrawn from the same clonal origin as the other three. As expected, no polymorphism was detected between the material propagated in vitro and the donor plants they originated from.read more
Citations
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Journal ArticleDOI
Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers
TL;DR: The results suggest that the culture conditions used for axillary branching proliferation are appropriate for clonal propagation of almond clone VII, as they do not seem to interfere with the integrity of the regenerated plantlets.
Journal ArticleDOI
Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay
TL;DR: The results confirmed the clonal fidelity of the tissue culture-raised S. chirayita plantlets and corroborated the fact that axillary multiplication is the safest mode for multiplication of true to type plants.
Journal ArticleDOI
Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
TL;DR: Results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and applicable for clonal propagation of banana var.
Journal ArticleDOI
Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers
TL;DR: Using a protocol for high rate shoot multiplication, certain morphological abnormalities observed during proliferation of shoot buds in vitro were not observed during acclimatization ex vitro, and the genetic stability of plantlets was assessed using RAPD and ISSR markers.
Book ChapterDOI
Somaclonal Variation in Tissue Culture: A Case Study with Olive
TL;DR: Studying somaclonal variation in micropropagation is important for its control and possible suppression with the aim of producing genetically identical plants, and for its use as a tool to produce genetic variability, which will enable breeders the genetic improvement.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Mathematical model for studying genetic variation in terms of restriction endonucleases
Masatoshi Nei,Wen-Hsiung Li +1 more
TL;DR: A mathematical model for the evolutionary change of restriction sites in mitochondrial DNA is developed and a measure called "nucleotide diversity" is proposed to express the degree of polymorphism in a population at the nucleotide level.
Journal ArticleDOI
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
Journal ArticleDOI
Development and differentiation of haploid Lycopersicon esculentum (tomato).
Peter M. Gresshoff,Colin H. Doy +1 more
TL;DR: Haploid callus cultures of selected races of Lycopersicon (tomato) species can be obtained from anther culture as a further demonstration of a proposed general method of haploid culture developed with Arabidopsis thaliana.
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