Recent Advancements in Tracking Bacterial Effector Protein Translocation
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TLDR
A comprehensive understanding of effector translocation in a spatio-temporal manner is of critical importance to gain insights into an effector’s mode of action.Abstract:
Bacteria-host interactions are characterized by the delivery of bacterial virulence factors, i.e., effectors, into host cells where they counteract host immunity and exploit host responses allowing bacterial survival and spreading. These effectors are translocated into host cells by means of dedicated secretion systems such as the type 3 secretion system (T3SS). A comprehensive understanding of effector translocation in a spatio-temporal manner is of critical importance to gain insights into an effector’s mode of action. Various approaches have been developed to understand timing and order of effector translocation, quantities of translocated effectors and their subcellular localization upon translocation into host cells. Recently, the existing toolset has been expanded by newly developed state-of-the art methods to monitor bacterial effector translocation and dynamics. In this review, we elaborate on reported methods and discuss recent advances and shortcomings in this area of tracking bacterial effector translocation.read more
Citations
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From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
TL;DR: In this article , different approaches are used to functionally characterize a type III secretion system (T3SS) through which effectors are translocated into the host cell, where they exercise a wide range of functions to deceive the host cells's immunity and to establish a niche.
Journal ArticleDOI
Bacterial Subcellular Architecture, Structural Epistasis, and Antibiotic Resistance
Fernando Baquero,José L. Martínez,Alvaro Sanchez,Miguel D Fernández-de-Bobadilla,Jerónimo Rodríguez-Beltrán +4 more
TL;DR: The concept of structural epistasis as mentioned in this paper was proposed to emphasize the role of variable physical interactions between molecules located in particular spaces inside the bacterial cell in the emergence of novel phenotypes.
Posted ContentDOI
Cell-based assay to determine Type 3 Secretion System translocon assembly in Pseudomonas aeruginosa using split luciferase
TL;DR: In this article , the split NanoLuc luciferase detection system was used to provide a readout for translocon assembly of Pseudomonas aeruginosa, which is an essential component of the Type III secretion system.
Journal ArticleDOI
Multitask Approach to Localize Rhizobial Type Three Secretion System Effector Proteins Inside Eukaryotic Cells
TL;DR: In this paper , the authors used a well-known rhizobial T3E, named NopL, to illustrate by a multitask approach where it localizes in heterologous hosts models, such as tobacco plant leaf cells, and also for the first time in transfected and/or Salmonella-infected animal cells.
Journal ArticleDOI
BopN is a Gatekeeper of the Bordetella Type III Secretion System
TL;DR: In this article , the crystal structure of BopN of B. pertussis and B. parapertussis was solved and it was shown that it is similar to the structures of gatekeepers that control access to the T3SS channel from the bacterial cytoplasm.
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