Showing papers in "Analytical Biochemistry in 2002"

TL;DR: In this paper, compositions and methods for increasing the intrinsic fluorescence intensity of biomolecules and low quantum yield fluorophores are described. And methods for the identification of nucleic acids are also provided.

TL;DR: The results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies.

TL;DR: Based on the simulation of kinetic process of real-time PCR, a new method for quantitation and normalization of gene transcripts is developed that provides a simple and accurate approach to quantifying gene expression level with the advantages that neither construction of standard curve nor validation experiments are needed.

TL;DR: The present method provides a simple, cost-effective assay for the estimation of stable oxidation products of nitric oxide in biological samples.

TL;DR: In this article, the authors report the separation and analysis of highly polar compounds using hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization (ESI) mass spectrometry.

TL;DR: A method for the simultaneous measurement of arginine, ADMA, and SDMA is reported on as a tool to gain insight in the role of these compounds in the regulation of NOS activity and is a valuable tool in research on the metabolism of dimethylated arginines.

TL;DR: The results indicate the need to test each antibody to be used on an antibody array and to select the microarray coating based on experimental requirements.

TL;DR: An improved two-step real-time RT-PCR procedure using SYBR green I and the LightCycler that better permits accurate quantification of mRNAs and the choice of the cDNA priming oligo can improve detection sensitivity even further.

TL;DR: In vivo application is demonstrated by using Oxyphor G2 to noninvasively determine the oxygen distribution in a subcutaneous tumor growing in Rats by using the calibration constants of the phosphors essentially independent of pH in the physiological range.

TL;DR: A new method for measurement of free malondialdehyde in human serum is described, fast and practical for clinical measurements, and patients with goiter had high levels of MDA compared to control groups.

TL;DR: Clear transformation of the patterns was observed in the case of treatment by the recombinant hormone, suggesting that this method can be regarded an efficient tool for indicating recombinant EPO misuse in sports.

TL;DR: A novel renewable reagentless hydrogen peroxide (H(2)O(2)) sensor based on the direct electron transfer of horseradish peroxidase (HRP) is proposed and displayed an excellent electrocatalytic response to the reduction of H(2), without the aid of an electron mediator.

TL;DR: A reverse transcriptase PCR (RT-PCR) assay is optimized and validated for accurate expression profiling using the double stranded DNA-binding dye SYBR green I, which is a much more economical alternative to quantify any given transcript in a reaction.

TL;DR: In all applications, the desthiobiotin/biotin-binding protein complex is easily dissociated under physiological conditions by either biotin or desthiOBiotin, so these reagents and techniques provide some distinct advantages over traditional 2-iminobiot in, monomeric avidin, or other affinity-based techniques.

TL;DR: A more sensitive fluorescent assay for lysyl oxidase activity that utilizes 1,5-diaminopentane as substrate, and released hydrogen peroxide is detected using Amplex red in horseradish peroxidase-coupled reactions is described.

TL;DR: Positions of the Soret absorbance band suggest that Mb and Hb in CS films keep their secondary structure similar to the native states in the medium pH range, while HRP and Ct retain their native conformation at least in the dry CS films.

TL;DR: This new technology takes advantage of the very high sensitivity of matrix-assisted laser desorption/ionization mass spectrometry and is implemented on a commercial instrument with modifications to only a few components and the software.

TL;DR: The main focus of the review is on the design and application of fluorescent probes as analytical tools for assessing labile iron and iron transport mechanisms and the efficiency of iron chelators in solution and in cellular systems.

TL;DR: A convenient single-step boiling deionized water (DW) method for extracting cellular ATP to replace perchloric acid and Tris-borate buffer that was precise for both suspended cells and attached cells and was effective in inhibiting ATPase.

TL;DR: A sensitive, rapid, quantitative strategy has been developed for O-glycan analysis that provides a basis for the analysis of subpicomole quantities of O- Glycans from normal levels of natural glycoproteins.

TL;DR: Luciferases are better reporters in whole-cell sensor bacteria and fluorescent proteins, GFP and DsRed, gave responses at higher analyte concentrations and after significantly longer incubation times.

TL;DR: A rapid, simple, and reliable method has been developed for the characterization and quantitation of ceramide molecular species directly from chloroform extracts of biological samples by electrospray ionization tandem mass spectrometry (ESI/MS/MS).

TL;DR: It is shown that PACE is a sensitive and simple tool for studying the monosaccharide composition of polysaccharides and of cell wall preparations and in combination with specific hydrolases, it can be used to analyze the structure of polySaccharides.

TL;DR: The ferrous oxidation in xylenol orange (FOX) assay for hydroperoxides suffers from very narrow pH optimum in the range 1.7-1.8, and substitution of perchloric for the sulfuric acid resulted in a lowering of the optimum pH of the assay to 1.1, a decreased dependence of the absorbance of the ferric-xylenl orange complex on acid concentration and decreased sensitivity to added compounds.

TL;DR: Pressure perturbation calorimetry is a new technique that measures the heat change in a solution that results when the pressure above the solution is changed, finding that ligand binding to ribonuclease A led to changes in alpha suggesting a burial of some surface area in the ligand-protein complex.

TL;DR: Two recombinant His-tagged proteins were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography, providing a reliable and facile method to produce these delicate biological intermediates.

TL;DR: This multivalent assay was able to reliably detect, in these same multivalent extracts, expressed mRNA as well as specific cellular and extracellular matrix proteins, which could be applied to a variety of cells cultured in biopolymers and to tissues from which biochemical components may be otherwise difficult to extract.

TL;DR: HPTLC can be used to study the phospholipid composition of cell-derived microparticles and may also be a useful technique for the analysis of other samples that are available only in minor quantities.

TL;DR: The reported mass spectrometric method has the advantages of speed, directness, sensitivity, and ease over protein sequencing and Western-blotting methods and holds the promise of an improved method for determining the status of histone modifications in the field of chromosome research.