Journal ArticleDOI
'Second-look' cytotoxicity: an evaluation of culture plus cytotoxin assay of Clostridium difficile isolates in the laboratory diagnosis of CDAD
Emilio Bouza,Teresa Peláez,Rodolfo Hernández Alonso,Pilar Catalán,Patricia Muñoz,M. Rodriguez Creixems +5 more
TLDR
The combination of direct-cell culture assay, culture for toxigenic C. difficile and "second-look" cell culture assay enhances the potential for diagnosis of CDAD and enables us to be more efficient with patient care resources.About:
This article is published in Journal of Hospital Infection.The article was published on 2001-07-01. It has received 64 citations till now. The article focuses on the topics: Clostridium difficile & Cell culture assays.read more
Citations
More filters
Journal Article
Risk factors for Clostridium difficile carriage and C. difficile-associated diarrhea in a cohort of hospitalized patients
TL;DR: C. difficile was a common Nosocomial infection on this ward, resulting in asymptomatic carriage more often than diarrhea and accounting for one-fifth of all cases of nosocomial diarrhea.
Journal ArticleDOI
Differences in outcome according to Clostridium difficile testing method: a prospective multicentre diagnostic validation study of C difficile infection
Tim Planche,Kerrie Davies,Pietro G Coen,John Finney,Irene M. Monahan,K. Morris,Lily O’Connor,Sarah Oakley,Cassie F Pope,Mike W Wren,Nandini Shetty,Derrick W. Crook,Mark H. Wilcox +12 more
TL;DR: A new diagnostic category of potential C difficile excretor (cytotoxigenic culture positive but cytotoxin assay negative) could be used to characterise patients with diarrhoea that is probably not due to C diffICile infection, but who can cause cross-infection.
Journal ArticleDOI
Comparison of Nine Commercially Available Clostridium difficile Toxin Detection Assays, a Real-Time PCR Assay for C. difficile tcdB, and a Glutamate Dehydrogenase Detection Assay to Cytotoxin Testing and Cytotoxigenic Culture Methods
TL;DR: The optimum rapid single test was PCR for toxin B gene, as this had the highest negative predictive value and was the highest sensitivity of all the tests in comparison with CYT and CYTGC.
Journal ArticleDOI
Reassessment of Clostridium difficile Susceptibility to Metronidazole and Vancomycin
Teresa Peláez,Luis Alcalá,Rodolfo Hernández Alonso,Marta Rodríguez-Créixems,Juan M. García-Lechuz,Emilio Bouza +5 more
TL;DR: Rates of resistance to metronidazole and vancomycin were higher among isolates from human immunodeficiency virus-infected patients, and molecular typing methods proved the absence of clonality among the isolates with decreased susceptibilities to the antimicrobials tested.
Journal ArticleDOI
Biology of Clostridium difficile: Implications for Epidemiology and Diagnosis
TL;DR: The combination of more aggressive strains with mobile genomes in a setting of an expanded pool of individuals at risk has refocused attention on and challenged assumptions regarding diagnostic gold standards.
References
More filters
Book
Manual of clinical microbiology
TL;DR: A collaborative team of editors and authors from around the world revised the Manual to include the latest applications of genomics and proteomics, producing an authoritative work of two volumes filled with current findings regarding infectious agents, leading-edge diagnostic methods, laboratory practices, and safety guidelines.
Journal ArticleDOI
Nosocomial acquisition of Clostridium difficile infection
TL;DR: Nosocomial C. difficile infection, which was associated with diarrhea in about one third of cases, is frequently transmitted among hospitalized patients and that the organism is often present on the hands of hospital personnel caring for such patients.
Journal ArticleDOI
Selective and differential medium for isolation of Clostridium difficile.
TL;DR: A selective and differential agar medium that contains cycloserine, cefoxitin, fructose, and egg yolk (CCFA) was developed to facilitate the isolation of C. difficile from fecal specimens and was found to be the most sensitive and selective of these media for the recovery of the bacterium.
Journal ArticleDOI
Clostridium difficile-associated diarrhea and colitis
TL;DR: The status of diagnosis, epidemiology, infection control, and treatment of Clostridium difficile -associated disease is reviewed and a case definition of CDAD is defined.
Journal ArticleDOI
Isolation of Clostridium difficile from the Environment and Contacts of Patients with Antibiotic-Associated Colitis
K.-H. Kim,Robert Fekety,Donald H. Batts,Donald W. Brown,Margaret Cudmore,Joseph Silva,D. Waters +6 more
TL;DR: C difficile was isolated from hands and stools of asymptomatic hospital personnel, from sewage and soil, and from the home of a patient, and environmental isolates were toxigenic.