Separation of normal and immune lymphoid cells by antigen-coated columns : antigen-binding characteristics of membrane antibodies as analyzed by hapten-protein antigens
Hans Wigzell,O. Mäkelä +1 more
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TLDR
The results strongly suggest that the potential humoral antibody-forming cell has preformed receptors on its outer surface with binding characteristics, indicating similarity, if not identity, to those of the eventual product of the cell, the humoral antibodies.Abstract:
Specific fractionation of immunologically competent cells derived from normal or immune animals was achieved by filtration through antigen-coated bead columns. This selective retention of the relevant reactive cells could be shown to produce a cell population, which after passage through the column would behave like a suspension rendered immunologically tolerant by "classical" means. The immunologically specific elimination of potential antibody-forming capacity of the filtered cells could be shown to affect the IgM and the IgG system to a similar extent. Analysis of the binding characteristics of the membrane antibodies responsible for this selective retention indicate striking similarities to those of the humoral antibodies produced against the antigen. Thus, the surface receptor could distinguish isolated haptenic groups on a "foreign" carrier background and the receptor of the hapten-reactive cells in the present system (high-rate antibody-forming cells against NIP) failed to combine with carrier specific determinants in analogy with the binding behavior of the serum anti-hapten antibodies. The binding of anti-hapten reactive cells to bead-attached haptens could be specifically inhibited by the presence of free hapten in the columnar fluid during cellular filtration. The results strongly suggest that the potential humoral antibody-forming cell has preformed receptors on its outer surface with binding characteristics, indicating similarity, if not identity, to those of the eventual product of the cell, the humoral antibody.read more
Citations
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Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells
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The regulatory influence of activated T cells on B cell responses to antigen.
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Membrane receptors and hormone action.
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Carrier function in anti-hapten antibody responses : iii. stimulation of antibody synthesis and facilitation of hapten-specific secondary antibody responses by graft-versus-host reactions
TL;DR: It is suggested that the allogeneic cells function by virtue of a specific immunologic attack of grafted cells on host cells, and may offer a key to the delineation of mechanisms involved in the activation of precursors of antibody-forming cells.
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Carrier function in anti-hapten antibody responses. IV. Experimental conditions for the induction of hapten-specific tolerance or for the stimulation of anti-hapten anamnestic responses by "nonimmunogenic" hapten-polypeptide conjugates.
TL;DR: Administration of nonimmunogenic 2,4-dinitrophenyl (DNP) conjugates of copolymers of D or L-glutamic acid and lysine (GL) induces hapten-specific tolerance in nonimmune and DNP-ovalbumin-primed strain 13 guinea pigs.
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