Journal ArticleDOI
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
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Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.Abstract:
RECENT procedures for the immunological assay of protein hormones in human plasma1–3 require the routine preparation of hormones labelled with iodine-131 of high specific activity.read more
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Journal ArticleDOI
The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
Journal ArticleDOI
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
Eva Engvall,Peter Perlmann +1 more
Journal ArticleDOI
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay
A. E. Bolton,William M. Hunter +1 more
TL;DR: With some antisera the immunoreactivity of the antigen was diminished by the introduction of a single I atom into the tyrosyl groups, whereas antigen containing a single (125)I-labelled 3-(4-hydroxyphenyl)propionamide group showed the same immunore activity as the unmodified antigen.
Journal ArticleDOI
Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product
Donald P. Bottaro,Jeffrey S. Rubin,D. L. Faletto,Andrew K Chan,Thomas E. Kmiecik,G F Vande Woude,Stuart A. Aaronson +6 more
TL;DR: A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase.
Journal ArticleDOI
High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis.
Birgit Millauer,Susanne Wizigmann-Voos,Harald Schnürch,Ricardo Martinez,Niels Møller,Werner Risau,Axel Ullrich +6 more
TL;DR: Investigation of flk-1 receptor tyrosine kinase mRNA expression by in situ hybridization analysis revealed specific association with endothelial cells at all stages of mouse development, suggesting a major role of this ligand-receptor signaling system in vasculogenesis and angiogenesis.
References
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Journal ArticleDOI
Immunoassay of Endogenous Plasma Insulin in Man
TL;DR: For years investigators have sought an assay for insulin which would combine virtually absolute specificity with a high degree of sensitivity, sufficiently exquisite for measurement of the minute insulin concentrations usually present in the circulation as mentioned in this paper.
Journal ArticleDOI
Studies on human growth hormone. i. a radio-immunoassay for human growth hormone
Journal ArticleDOI
Glucagon Antibodies and Their Use for Immunoassay for Glucagon.
Roger H Unger,Anna M. Eisentraut,Mary Sue McCall,Sue Keller,Henry C. Lanz,Leonard L. Madison +5 more
TL;DR: Non-precipitating antibodies to glucagon have been produced in rabbits and detected by both Berson-Yalow and Skom-Talmadge technics, and a basis for an immunoassay for glucagon appears to have been established.
Journal ArticleDOI
Trace-labelling of insulin with iodine.
Ellis Samols,H. S. Williams +1 more
TL;DR: A new technique for the immunological assay of human plasma insulin requires a radioactive bovine insulin of very high specific activity to be used.