Serotyping and esterase typing for analysis of Listeria monocytogenes populations recovered from foodstuffs and from human patients with listeriosis in Belgium.
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The secretion of the virulence determinant phosphatidylinositol-specific phospholipase C and the pathogenicity level of strains in immunocompromised mice could not explain the unequal distribution of esterase types.Abstract:
Listeria monocytogenes strains isolated in Belgium from different foodstuffs and in sporadic cases of human listeriosis were analyzed. The distribution of serovars differed in each of these populations. The bacteria isolated from cheeses and from human patients with listeriosis were further studied by esterase typing. The twenty esterase patterns defined were not equally distributed in these two populations. The secretion of the virulence determinant phosphatidylinositol-specific phospholipase C and the pathogenicity level of strains in immunocompromised mice could not explain the unequal distribution of esterase types. The discrimination index of esterase typing (DI = 0.868) was compared with that of serotyping (DI = 0.666) and with that of the two combined methods (DI = 0.899).read more
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Listeria monocytogenes, a food-borne pathogen
TL;DR: This article corrects the article on p. 485 in vol.
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Listeriosis outbreaks and associated food vehicles, United States, 1998-2008.
Emily J. Cartwright,Kelly A. Jackson,Shacara D. Johnson,Lewis M. Graves,Benjamin J. Silk,Barbara E. Mahon +5 more
TL;DR: Outbreak investigations can identify industrial gaps and regulatory measures to protect food that need to be considered in the context of an outbreak.
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Correlations between Molecular Subtyping and Serotyping of Listeria monocytogenes
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Listeria monocytogenes Serotype Identification by PCR
TL;DR: Three primer sets were used in conjunction with a previously described Division III primer set in order to classify 122 L. monocytogenes strains into five serotype groups, and results of the PCR method agreed with those of the conventional slide agglutination method for 97, 100, 94, and 91% of strains belonging to serotypes 1/2a, 1/ 2b,1/2c, and 4b, respectively.
References
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Lowell J. Reed,H. Muench +1 more
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Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.
Paul R. Hunter,M A Gaston +1 more
TL;DR: An index of discrimination for typing methods is described, based on the probability of two unrelated strains being characterized as the same type, which may be used to compare typing methods and select the most discriminatory system.
Journal ArticleDOI
Listeria monocytogenes, a food-borne pathogen.
Jeffrey M. Farber,P I Peterkin +1 more
TL;DR: Improved methods for detecting and enumerating the organism in foodstuffs are now available, including those based on the use of monoclonal antibodies, DNA probes, or the polymerase chain reaction, which can help in the prevention and control of human infection.
Listeria monocytogenes, a food-borne pathogen
TL;DR: This article corrects the article on p. 485 in vol.