Significance of Reactive Oxygen Species and Antioxidants in Defining the Efficacy of Sperm Preparation Techniques
R. John Aitken,Jane S. Clarkson +1 more
Reads0
Chats0
TLDR
Assessment of the ability of the antioxidants, butylated hydroxytoluene, and vitamin E, to curtail the peroxidative damage inflicted by cells in response to centrifugation revealed a significant improvement of sperm function in the presence of vitamin E.Abstract:
The mechanisms responsible for mediating the influence of sperm preparation protocols on human sperm function have been investigated. Techniques that involved the separation of motile spermatozoa prior to centrifugation were found to yield sperm suspensions of highest quality. If the spermatozoa were centrifuged prior to isolation of the motile cells, sperm function was impaired. The detrimental effects of centrifugation were associated with a sudden burst of reactive oxygen species production by a discrete subpopulation of cells (characterized by significantly diminished motility and fertilizing capacity) that could be separated from normal functional spermatozoa on Percoll gradients. If unfractionated sperm suspensions were subjected to centrifugation, the reactive oxygen species generated by this subpopulation impaired the functional competence of normal spermatozoa in the same suspension. Assessment of the ability of the antioxidants, butylated hydroxytoluene, and vitamin E, to curtail the peroxidative damage inflicted by such cells in response to centrifugation revealed a significant improvement of sperm function in the presence of vitamin E.read more
Citations
More filters
Journal ArticleDOI
Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique
Giuseppe Ricci,Sandra Perticarari,Rita Boscolo,Marcella Montico,Secondo Guaschino,Gianni Presani +5 more
TL;DR: Both sperm preparation methods allow obtaining a sperm population with a low percentage of apoptotic sperm, which seems to be rather low and depends on whether IVF/ICSI or intrauterine insemination is to be performed.
Journal ArticleDOI
Scavenging effect of N-acetyl-L-cysteine against reactive oxygen species in human semen: a possible therapeutic modality for male factor infertility?
TL;DR: A new approach to reduce the level of reactive oxygen species (ROS) in human semen by using N‐acetyl‐L‐cysteine (NAG) was evaluated and the influence of NAC on functional sperm parameters (motility and acrosome reaction) was studied.
Journal ArticleDOI
Shorter abstinence decreases sperm deoxyribonucleic acid fragmentation in ejaculate
Jaime Gosálvez,Mercedes González-Martínez,Carmen López-Fernández,José Luis Fernández,Pascual Sánchez-Martín +4 more
TL;DR: The present results challenge the role of abstinence in current male infertility treatments and suggest that SDF can be efficiently reduced by a biological practice consisting of short-term recurrent ejaculation coupled with effective sperm selection.
Journal ArticleDOI
The role of antioxidant therapy in the treatment of male infertility: an overview.
Francesco Lombardo,Andrea Sansone,Francesco Romanelli,Donatella Paoli,Loredana Gandini,Andrea Lenzi +5 more
TL;DR: Since there is no linear correlation between sperm quality and pregnancy rates, an improvement in semen parameters should not be the sole outcome considered in studies of antioxidant therapies, and more multicentre, double-blind studies performed with the same criteria are necessary for an increased understanding of the effects of various antioxidants on fertility.
Journal ArticleDOI
Improvement in motion characteristics and acrosome status in cryopreserved human spermatozoa by swim-up processing before freezing
TL;DR: Selecting a highly motile sperm population before freezing enhances overall post-thaw spermatozoa quality and reduces the deleterious effects of cryopreservation.
References
More filters
Journal ArticleDOI
Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa.
TL;DR: Studies with scavengers of reactive oxygen species revealed that, while reagents directed against singlet oxygen and the hydroxyl radical were without effect, cytochrome C reduced the response to A23187 by about 50%, suggesting that the superoxide anion radical is a major product of the activated human spermatozoon.
Journal ArticleDOI
Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity.
TL;DR: The results suggest that superoxide dismutase plays the major role in protecting human spermatozoa against lipid peroxidation, and the superoxide Dismutase activity of a fresh sperm sample appears to be a good predictor of the lifetime (up to the complete loss of motility) of that particular sample, and so may prove useful in semen analysis.
Journal ArticleDOI
The Use of Zona-Free Animal Ova as a Test-System for the Assessment of the Fertilizing Capacity of Human Spermatozoa
TL;DR: The results suggest that zona-free hamster ova can be substituted for human ova in the preliminary assessment of the fertilizing capacity of human spermatozoa.
Journal ArticleDOI
Peroxidative Breakdown of Phospholipids in Human Spermatozoa, Spermicidal Properties of Fatty Acid Peroxides, and Protective Action of Seminal Plasma
TL;DR: The antiperoxidant factor present in human seminal plasma effectively counteracts the toxic effect of exogenous peroxidized fatty acids upon human spermatozoa, but is unable to restore motility lost by lipid peroxide action.