Journal ArticleDOI
Simplified and improved somatic embryogenesis for clonal propagation of Pinus pinaster (Ait.).
TLDR
Improvements and simplifications of SE protocols in Pinus pinaster (Ait), a species of economic importance in the regions of Western Europe, are described including high initiation frequencies in eight control pollinated seed families, relatively high somatic embryo maturation yield when cells were coated with particles of activated charcoal and a rapid production of plants directly in a shade house.Abstract:
In this study, several improvements and simplifications of SE protocols in Pinus pinaster (Ait.), a species of economic importance in the regions of Western Europe, are described. These improvements pertained to all stages of SE including high initiation frequencies in eight control pollinated seed families, relatively high somatic embryo maturation yield when cells were coated with particles of activated charcoal and a rapid production of plants directly in a shade house. The SE initiation frequency from isolated zygotic embryos was high (up to 100%) and plants were produced from 11 embryogenic lines representing all crosses. Based on these results, the estimated number of somatic embryos required to produce 1,000 plants varied from slightly more than the required number of plants to more than double this number depending on the line. Such an estimate is critical in developing plant production strategy when a number of embryogenic lines are considered for production of clonal plants.read more
Citations
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Journal ArticleDOI
The role of activated charcoal in plant tissue culture.
TL;DR: The effect of AC on growth regulator uptake is still unclear but some workers believe that AC may gradually release certain adsorbed products, such as nutrients and growth regulators which become available to plants.
Journal ArticleDOI
Somatic embryogenesis in forestry with a focus on Europe: state-of-the-art, benefits, challenges and future direction
Marie-Anne Lelu-Walter,David Thompson,Luc Harvengt,Leopoldo Sanchez,Mariano Toribio,Luc E. Pâques +5 more
TL;DR: This review will describe benefits, type of vegetative propagation, and its integration into breeding programmes, and a strategy to promote the use of somatic embryogenesis is proposed.
Journal ArticleDOI
De novo assembly of maritime pine transcriptome: implications for forest breeding and biotechnology.
Javier Canales,Rocío Bautista,Philippe Label,Josefa Gómez-Maldonado,Isabelle Lesur,Isabelle Lesur,Noe Fernandez-Pozo,Marina Rueda-López,Darío Guerrero-Fernández,Vanessa Castro-Rodríguez,Hicham Benzekri,Rafael A. Cañas,María Angeles Guevara,Andreia S. Rodrigues,Pedro Seoane,Caroline Teyssier,Alexandre Morel,François Ehrenmann,François Ehrenmann,Grégoire Le Provost,Grégoire Le Provost,Céline Lalanne,Céline Lalanne,Céline Noirot,Christophe Klopp,Isabelle Reymond,Angel García-Gutiérrez,Jean-François Trontin,Marie-Anne Lelu-Walter,Célia Miguel,María Teresa Cervera,Francisco R. Cantón,Christophe Plomion,Christophe Plomion,Luc Harvengt,Concepción Ávila,M. Gonzalo Claros,Francisco M. Cánovas +37 more
TL;DR: Detailed characterization of the maritime pine transcriptome was performed using a combination of two different next-generation sequencing platforms, 454 and Illumina, allowing the identification of polymorphisms and the establishment of robust single nucleotide polymorphism (SNP) and simple-sequence repeat (SSR) databases for genotyping applications and integration of translational genomics in maritime pine breeding programmes.
Recent Progress in Somatic Embryogenesis of Four Pinus spp.
Krystyna Klimaszewska,Jean-François Trontin,Michael R. Becwar,Christine Devillard,Y. S. Park,Marie-Anne Lelu-Walter +5 more
TL;DR: This review emphasizes the most recent progress made in SE and cryopreservation in the genus Pinus, focusing on major plantation and forest species in Europe, New Zealand, Australia, and South America, as well as North America.
Book ChapterDOI
Advances in Conifer Somatic Embryogenesis Since Year 2000.
TL;DR: This review compiles research results published over the last 14 years on conifer somatic embryogenesis, with emphasis on the newest findings that affect the response of seed embryos and shoot primordia to the induction of SE and long-term culture of early somatic embryos.
References
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Book
Generalized Linear Models
Peter McCullagh,John A. Nelder +1 more
TL;DR: In this paper, a generalization of the analysis of variance is given for these models using log- likelihoods, illustrated by examples relating to four distributions; the Normal, Binomial (probit analysis, etc.), Poisson (contingency tables), and gamma (variance components).
Book
Applied Mixed Models in Medicine
Helen Brown,Robin J Prescott +1 more
TL;DR: Applied mixed models in medicine, Applied mixed model in medicine , کتابخانه دیجیتال جندی شاپور اهواز
BookDOI
Somatic embryogenesis in woody plants
TL;DR: This work focuses on Somatic Embryogenesis in African Cycads (Encephalartos) and its application to Clonal Forestry, as well asCryopreservation of Embryogenic Cultures of Conifers and Its Application toclonal Forestry.
Journal ArticleDOI
Influence of a loblolly pine (Pinus taeda L.). Culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.).
TL;DR: A new culture medium, originally designed and shown to grow cell suspensions from a variety of loblolly pine explants, was used to study growth and somatic embryogenesis of the wild carrot in cell suspensions, and growth and embryogenesis in LM was superior to WCM.
Related Papers (5)
Influence of a loblolly pine (Pinus taeda L.). Culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.).
Shoot multiplication from mature trees of Douglas-fir ( Pseudotsuga menziesii ) and sugar pine ( Pinus lambertiana )
Pramod K. Gupta,Don J. Durzan +1 more