Journal ArticleDOI
Single primer amplification reaction (SPAR) reveals inter- and intra-specific natural genetic variation in five species of Cymbidium (Orchidaceae).
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TLDR
The present investigation for the first time provides a valuable baseline data for genetic variation at inter- and intra-specific levels in horticultural Cymbidiums and also addresses conservation concerns.About:
This article is published in Gene.The article was published on 2011-09-01. It has received 36 citations till now. The article focuses on the topics: RAPD & Genetic distance.read more
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Applicability of ISSR and DAMD markers for phyto-molecular characterization and association with some important biochemical traits of Dendrobium nobile, an endangered medicinal orchid
TL;DR: A high degree of existing genetic and phytochemical variation amongst the populations in relation to bioclimatic and geographic locations of populations is detected, and the cumulative marker approach could be the best suited for assessing the genetic relationships with high accuracy amongst distinct D. nobile accessions.
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Efficacy of RAPD, ISSR and DAMD markers in assessment of genetic variability and population structure of wild Musa acuminata colla
Animos Lamare,Satyawada Rama Rao +1 more
TL;DR: The study provides insight into the efficacy of RAPD, ISSR and DAMD to analyse the genetic variation existing in the wild Musa germplasm, which can further be exploited for quality trait improvement and domestication of such important horticultural crops.
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Assessment of phylogenetic inter-relationships in the genus Cymbidium (Orchidaceae) based on internal transcribed spacer region of rDNA.
TL;DR: This study validates the utility of ITS rDNA region as a reliable indicator of phylogenetic relationships, especially ITS 2 as probable DNA barcode at higher levels and can serve as an additional approach for identification of broader range of plant taxa especially orchids.
Journal ArticleDOI
Molecular characterization of Dendrobium nobile Lindl., an endangered medicinal orchid, based on randomly amplified polymorphic DNA
TL;DR: The data represented in this study suggested that the RAPD method was a valuable tool for estimation of genetic diversity and genetic relatedness of the D. nobile germplasm.
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Isolation of genomic DNA suitable for community analysis from mature trees adapted to arid environment.
TL;DR: In this article, a standardized protocol for the isolation and purification of genomic DNA (gDNA) from seven ecologically and medically important tree species of Combretaceae viz. Anogeissus, Terminalia (Terminalia arjuna), Terminalia catappa and Terminalia chebula), was reported.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
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Rapid isolation of high molecular weight plant DNA
TL;DR: A method is presented for the rapid isolation of high molecular weight plant DNA which is free of contaminants which interfere with complete digestion by restriction endonucleases, and which yields total cellular DNA.
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Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
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POPULATION GENETIC CONSEQUENCES OF SMALL POPULATION SIZE: Implications for Plant Conservation
TL;DR: The effects of genetic drift, inbreeding, and gene flow on genetic diversity and fitness in rare plants and small populations and those circumstances that are likely to put these plant species and populations at genetic risk are identified.