Journal ArticleDOI
Sister Chromatid Exchanges in Lymphocytes in Operating Room Personnel
B. Husum,H. C. Wulf +1 more
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TLDR
Sister chromatid exchange points were counted in lymphocytes in peripheral blood drawn from hospital personnel exposed to anaesthetics as well as from persons not exposed.Abstract:
Sister chromatid exchanges (SCE) and sister chromatid exchange points (SCE-points) were counted in lymphocytes in peripheral blood drawn from hospital personnel exposed to anaesthetics as well as from persons not exposed.
A total of 38 healthy persons were investigated, representing female nurse anaesthetists, male physicians practising anaesthesia, female nurses from the intensive care unit, and female secretaries.
The mean SCE number per cell for each person was used as the variable, and the Mann-Whitney U-test was applied to test for differences between groups. The group of secretaries seemed to differ from the other three groups, which appeared identical (P<0.001). Correlation of cigarette smoking and number of SCE could not be demonstrated (r=.255, n = 38).
It was concluded that by this method there was no indication of a mutagen effect of long-term exposure to waste anaesthetic gases such as halothane and nitrous oxide.read more
Citations
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Journal ArticleDOI
Genotoxicity of inhalation anesthetics halothane and isoflurane in human lymphocytes studied in vitro using the comet assay
TL;DR: The increase of DNA migration induced in PBL by isoflurane at 1 mM and by halothane at 0.1 mM was not a result of cell death-associated DNA degradation but was caused by genotoxic action of the drugs.
Journal ArticleDOI
Genetic damage in operating room personnel exposed to isoflurane and nitrous oxide.
Klaus Hoerauf,Michael Lierz,G. Wiesner,Klaus Schroegendorfer,Peter Lierz,A. Spacek,Leo Brunnberg,Michael Nüsse +7 more
TL;DR: Exposure even to trace concentrations of waste anaesthetic gases may cause dose-dependent genetic damage and the increased proportion of micronuclei and rates of sister chromatid exchanges may be relevant long term and need further investigation.
Journal ArticleDOI
Metallurgy in Ancient Eastern Asia: Retrospect and Prospects
Katheryn M. Linduff,Jianjun Mei +1 more
TL;DR: The authors suggest that metallurgy emerged independently in a core area and then spread to peripheral areas by way of political expansion and cultural diffusion over many millennia, and that metheurgy was also homegrown.
Journal ArticleDOI
Sister-chromatid exchanges in operating room personnel
TL;DR: This study supports the existence of an association between occupational exposure to mutagens and an increase in SCEs in lymphocytes in operating room personnel.
References
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Journal ArticleDOI
Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test
TL;DR: The methods described include the standard plate test, the use and storage of the bacterial tester strains, preparation and use of the liver homogenates, and the methods of inducing the rats for elevated microsomal enzyme activity.
Journal ArticleDOI
New Giemsa method for the differential staining of sister chromatids
Paul Perry,Sheldon Wolff +1 more
TL;DR: If human lymphocytes1 or Chinese hamster2 cells are treated with the base analogue 5-bromodeoxyuridine in the latter part of the S period, Giemsa stained chromosomes exhibit a pattern of condensed and extended segments along their length, allowing the identification of the two chromatids, and the observation of sister chromatid exchanges (SCEs) without recourse to autoradiography.
Journal ArticleDOI
Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange.
Paul Perry,H. J. Evans +1 more
TL;DR: A staining technique that detects sister chromatid exchanges (SCEs) has been used to examine the response of chromosomes in cultured Chinese hamster cells to a wide variety of mutagens–carcinogens.
Journal ArticleDOI
Chromosome aberrations and sister-chromatid exchange in workers in chemical laboratories and a rotoprinting factory and in children of women laboratory workers.
F. Funes-Cravioto,B. Kolmodin-Hedman,J. Lindsten,M. Nordenskjöld,C. Zapata-Gayon,B. Lambert,E. Norberg,R. Olin,Å. Swensson +8 more
TL;DR: Cultured lymphocytes from 73 workers in chemical laboratories and the printing industry were found to have a significantly increased frequency of chromatid and isochromatid breaks, and a significant correlation between age and frequency of chromosome aberrations was noted for both the exposed and control children.
Journal ArticleDOI
Sister Chromatid Exchange and Chromosome Aberration Analysis With the Use of Several Carcinogens and Noncarcinogens: Brief Communication
TL;DR: No correlation was found between the frequencies of SCE and aberrations, but two chemicals, which are mutagenic in microorganisms but whose carcinogenicity is poorly documented, increased the frequency of S CE.
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