Journal ArticleDOI
Size, conformation and purity of chloroplast DNA of some higher plants.
TLDR
Electron microscopic analysis of chloroplast DNA, purified in CsCl or Cscl/ethidium bromide gradients, revealed up to 80% circular molecules, which are comparable to the size derived for the coding capacity of chloroplasts from reassociation experiments.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1975-01-20. It has received 115 citations till now. The article focuses on the topics: Chloroplast DNA & Nuclear DNA.read more
Citations
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Book ChapterDOI
Isolation and structural analysis of chloroplast DNA
TL;DR: This chapter describes a filter hybridization approach for mapping chloroplast DNA (cpDNA) restriction sites along with several strategies for generating restriction fragments for use as hybridization probes.
Journal ArticleDOI
The molecular size and conformation of the chloroplast DNA from higher plants.
Richard D. Kolodner,K.K. Tewari +1 more
TL;DR: Covalently closed circular choloroplast DNA (ctDNA) molecules have been isolated from pea, bean, spinach, lettuce, corn and oat plants by ethidium bromide/cesium choloride density-gradient entrifugation.
Journal ArticleDOI
The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide sequence and gene organization
Christian Schmitz-Linneweber,Rainer M. Maier,Jean-Pierre Alcaraz,Annick Cottet,Reinhold G. Herrmann,Régis Mache +5 more
TL;DR: The chloroplast chromosome of spinach is a double-stranded circular DNA molecule of 150 725 nucleotide pairs and a comparison of this chromosome with those of the three other autotrophic dicotyledons for which complete DNA sequences of plastid chromosomes are available confirms a conserved overall structure.
Journal ArticleDOI
Circular Chloroplast Chromosomes: The Grand Illusion
TL;DR: If the authors could extract, purify, and visualize the intact DNA molecules from chloroplasts, what would those molecules look like?
Journal ArticleDOI
Inverted repeats in chloroplast DNA from higher plants
Richard D. Kolodner,K. K. Tewari +1 more
TL;DR: It is proposed that the head-to-head circular dimers are formed by a recombination event between two circular monomers in the inverted sequence.
References
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Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
TL;DR: A comprehensive study of the buoyant density of DNA as a function of composition has been made and the linear relation previously reported has been confirmed.
Journal ArticleDOI
Molecular weights of coliphages and coliphage DNA: IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M☆
TL;DR: The possibility that Ml is not constant for all DNA molecules is discussed and using sedimentation data an equation relating S20,w0 and M for bulk sedimentation and a second equation for sedimentation in sucrose gradients are presented.
Journal ArticleDOI
Density-Gradient centrifugation of DNA in a fixed-angle rotor: A higher order of resolution
W.G. Flamm,H.E. Bond,H.E. Burr +2 more
TL;DR: The data presented in this paper show that the fixed-angle rotor offers from 5 to 10-fold greater resolution than the swinging-bucket rotor and that it has a capacity per tube of at least 10 times that of the swingside rotor.
Journal ArticleDOI
Molecular weights of coliphages and coliphage DNA. 3. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus.
TL;DR: Based on the mean value of 25.1 × 10 6 daltons, obtained from three recent and independent determinations of T7 DNA molecular weight, the molar linear density of duplex DNA as prepared by standardized electron microscopy was found to be 2.07 × 10 10 for DNA with common bases, 2.28 × 1010 for T4 DNA, and 2.23 ×10 10 dalton/cm for T2 DNA.
Journal ArticleDOI
Darstellung und längenmessungen des gesamten desoxyribonucleinsäure-inhaltes von T2-bakteriophagen
TL;DR: Electron micrographs of DNA macromolecules are prepared by spreading a suitable protein-salt solution with T2 phages to a mixed monolayer on a water-air interface and the first data of an average T2-DNA length of 49±4 μ are discussed in relation to the possible sources of error.
Related Papers (5)
The molecular size and conformation of the chloroplast DNA from higher plants.
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