Journal ArticleDOI
Skeletal alkaline phosphatase activity as a bone formation index in vitro.
John R. Farley,David J. Baylink +1 more
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It was found that ALP activity and 3[H]-hyp incorporation were coordinately increased from pH 5.5 to pH 7.2 and high and low pH, and when the exposure to effectors was limited to a preincubation, or when the low [Ca] data were excluded, a correlation was observed.Abstract:
These studies were intended to examine the relationship between skeletal collagen formation and skeletal alkaline phosphatase (ALP) activity in vitro. Embryonic chick calvaria were exposed to skeletal effectors (including high and low pH, a range of [p i ] and [Ca], PTH, NaF, etc), and collagen formation was assessed by the incorporation of 3 [H]-proline as 3 [H]-hydroxyproline ( 3 [H]-hyp). ALP activity was measured in the serum-free conditioned medium and in 20% butanol extracts of the bones. We found that ALP activity and 3 [H]-hyp incorporation were coordinately increased from pH 5.5 to pH 7.2 ( r = .99, P i ], but low [Ca] increased ALP and coordinately decreased collagen formation ( r = −.81, P 3 [H]-hyp incorporation were coordinately increased by NaF, vanadate, PGE 2 , calcitonin, and insulin, the slopes of the correlations were not the same for all effectors (eg, NaF: r = .97, P r = .95, P 3 [H]-hyp incorporation. When a variety of effectors, including low [Ca], were used to treat different groups of calvaria, ALP activity was not correlated with 3 [H]-hyp incorporation ( r = .35), but when the exposure to effectors was limited to a preincubation, or when the low [Ca] data were excluded, a correlation was observed ( r = .87, P r = .64, P r = .60, P r = .89, P 3 [H]-hyp incorporation and (2) even under conditions in which that correlation is disrupted (ie, in the presence of specific skeletal effectors), skeletal ALP is still useful as a predictive index of the capacity for in vitro calvarial collagen synthesis (ie, the predicted rate of 3 [H]-hyp incorporation if the effectors were removed).read more
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Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways
TL;DR: It is shown for the first time that the signal transduction mechanism of shear stress in osteoblasts is complex and involves multiple ERK-dependent and independent pathways, and circumstantial evidence that there may be a PTX-sensitive pathway that has completing effects with an unknown pathway on the differentiation of normal human osteoblast.
Journal ArticleDOI
Bone regeneration is regulated by wnt signaling.
Jae-Beom Kim,Philipp Leucht,Philipp Leucht,Kentson Lam,Cynthia A. Luppen,Derk ten Berge,Roel Nusse,Jill A. Helms +7 more
TL;DR: The role of Wnt signaling in skeletal tissue regeneration is studied to provide a glimpse into normal development as well as insights into pathological conditions such as cancer.
Journal ArticleDOI
Melatonin stimulates proliferation and type I collagen synthesis in human bone cells in vitro.
TL;DR: The pineal secretory product melatonin reportedly regulates release of growth hormone in humans and prevents phototherapy-induced hypocalcemia in newborn rats, suggesting that melatonin affects bone metabolism as mentioned in this paper.
Journal ArticleDOI
ECM Compliance Regulates Osteogenesis by Influencing MAPK Signaling Downstream of RhoA and ROCK
TL;DR: It is established that one possible mechanism by which ECM rigidity regulates osteogenic differentiation involves MAPK activation downstream of the RhoA‐ROCK signaling pathway.
Melatonin stimulates proliferation and type I collagen synthesis in human bone cells in vitro / Osamu Nakade ... [et al.].
TL;DR: New evidence is provided thatmelatonin stimulates the proliferation and type I collagen synthesis in human bone cells in vitro, suggesting that melatonin may act to stimulate bone formation.
References
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Fluoride directly stimulates proliferation and alkaline phosphatase activity of bone-forming cells.
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Treatment of osteoporosis with fluoride, calcium, and vitamin D.
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Stimulation of bone formation by prostaglandin E2.
Yong S. Chyun,Lawrence G. Raisz +1 more
TL;DR: It is concluded that PGE2 has stimulatory effects on bone formation and can reverse the inhibitory effects of cortisol, which may be mediated in part by their ability to reduce the endogenous production of prostaglandins.
Journal ArticleDOI
The role of alkaline phosphatase in osteogenesis.
TL;DR: The role of alkaline phosphatase in osteogenesis has been investigated by histochemical techniques with particular attention to its relationship to phosphate metabolism and matrix elaboration, and evidence to suggest that cartilage matrix is utilized in the formation of bone matrix.