Journal ArticleDOI
Somatic embryogenesis in peanut: Influence of growth regulators and sugars
Susan Eapen,Leela George +1 more
TLDR
2,4-d was more effective than picloram for inducing embryogenesis from embryonal axes, and sucrose was the best for embryo induction and at 6% sucrose the highest frequency of responding cultures and average number of somatic embryos per explant were obtained.Abstract:
Somatic embryogenesis and plant regeneration were induced from immature embryonal axes and immature cotyledons of peanut (Arachis hypogaea L. fastigata type cv JLM-1). Influence of different auxins, cytokinins and sugars on somatic embryogenesis from immature cotyledon explants was also investigated. Among the different auxins tested, 2,4-dichlorophenoxyacetic acid (2,4-d) was most effective, producing the highest frequency of responding cultures and highest average number of somatic embryos per responding culture, while dicamba, picloram, indolepropionic acid, α-naphthaleneacetic acid, 2,4,4-trichlorophenoxypropionic acid and α-naphthoxyacetic acid were also effective for embryogenesis. Indolebutyric acid, indoleacetic acid, p-chlorophenoxyacetic acid and trichlorophenoxyacetic acid were not beneficial. Among the four cytokinins tested, zeatin slightly enhanced the frequency of somatic embryogenesis, while kinetin, 6-γ-γ-dimethylallylaminopurine and benzyladenine were relatively inhibitory. Among the different carbon sources tested, sucrose was the best for embryo induction and at 6% sucrose the highest frequency of responding cultures and average number of somatic embryos per explant were obtained. For inducing embryogenesis from embryonal axes, 2,4-d was more effective than picloram. Highest plant conversion frequency from somatic embryos was obtained in presence of dicamba or NAA and using cotyledon explants.read more
Citations
More filters
Journal ArticleDOI
Thidiazuron : A potent regulator of in vitro plant morphogenesis
TL;DR: Several reports indicate that TDZ may act through modulation of the endogenous plant growth regulators, either directly or as a result of induced stress, and several possibilities include the modification in cell membranes, energy levels, nutrient uptake, or nutrient assimilation.
Journal ArticleDOI
Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons
TL;DR: It appeared that TDZ induced somatic embryogenesis in peanut by influencing endogenous levels of both auxin and cytokinins, while TDZ itself was detected only in the cotyledons.
Journal ArticleDOI
Genotype, explant and position effects on organogenesis and somatic embryogenesis in eggplant (Solanum melongena L.)
TL;DR: Pusa Purple Long was found to be the most responsive genotype for regeneration of both adventitious shoots and somatic embryos among the cultivars andGenotype, explant and genotype-explant interaction had highly significant effects on both organogenesis and somatics embryogenesis with genotype exerting maximum effect on both these processes.
Journal ArticleDOI
Induction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.).
TL;DR: Pepper (cv. New Mexico — 6 and Rajur Hirapur) plants were regenerated from immature zygotic embryos via direct somatic embryogenesis using Murashige and Skoog's medium containing 2,4-D, thidiazuron, and a high concentration of sucrose.
Journal ArticleDOI
Somatic embryogenesis and organogenesis in peanut: The role of thidiazuron and N6-benzylaminopurine in the induction of plant morphogenesis
TL;DR: These results clearly demonstrate that seedlings treated with TDZ undergo a different morphological route of development than that induced by purine cytokinins.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis
TL;DR: Using callus derived from immature embryos, regeneration of viable plants was obtained in soybean cultures and confirmed the organogenic and embryogenic nature of the cultures, by demonstrating the formation of shoot buds and somatic embryos, respectively.
Journal ArticleDOI
Maturation and germination of somatic embryos as affected by sucrose and plant growth regulators in soybeans Glycine gracilis Skvortz and Glycine max (L.) Merr.
TL;DR: The efficiency of somatic embryo germination was as high as 77% from semi-wild soybean and 60–64% from cultivated soybeans, showing that the plant regeneration system developed in this study was efficient and practical.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more