Speckle-based volume holographic microscopy for optically sectioned multi-plane fluorescent imaging.
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TLDR
The design, implementation, and experimental image data demonstrating the proposed speckle-based volume holographic microscopy system's ability to simultaneously obtain wide-field, optically sectioned, and multi-depth resolved images of fluorescently labeled microspheres and tissue structures are presented.Abstract:
Structured illumination microscopy has been widely used to reconstruct optically sectioned fluorescence images in wide-field fashion; however, it still requires axial scanning to obtain multiple depth information of a volumetric sample. In this paper, a new imaging scheme, called speckle-based volume holographic microscopy system, is presented. The proposed system incorporates volumetric speckle illumination and multiplexed volume holographic gratings to acquire multi-plane images with optical sectioning capability, without any axial scanning. We present the design, implementation, and experimental image data demonstrating the proposed system’s ability to simultaneously obtain wide-field, optically sectioned, and multi-depth resolved images of fluorescently labeled microspheres and tissue structures.read more
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References
More filters
Journal ArticleDOI
Coupled wave theory for thick hologram gratings
TL;DR: In this paper, a coupled wave analysis of the Bragg diffraction of light by thick hologram gratings is given, analogous to Phariseau's treatment of acoustic gratings and to the dynamical theory of X-ray diffraction.
Journal ArticleDOI
Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.
TL;DR: This work developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development to derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event.
Journal ArticleDOI
Fluorescence microscopy today.
TL;DR: The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
Journal ArticleDOI
Optical sectioning microscopy.
TL;DR: The core concepts of confocal microscopes and important variables that adversely affect confocal images are described and computational optical sectioning techniques that can perform Optical sectioning without a confocal microscope are discussed.
Journal ArticleDOI
Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy
Philipp J. Keller,Annette D. Schmidt,Anthony Santella,Khaled Khairy,Zhirong Bao,Joachim Wittbrodt,Joachim Wittbrodt,Ernst H. K. Stelzer +7 more
TL;DR: This method discriminates the specimen-related scattered background from signal fluorescence, thereby removing out-of-focus light and optimizing the contrast of in-focus structures, and provides rapid control of the illumination pattern, exceptional imaging quality and high imaging speeds.
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