Stimulation of glucagon of in vivo phosphorylation of rat hepatic pyruvate kinase.
TLDR
Rat hepatic pyruvate kinase (type L) has been purified to homogeneity by a simple, rapid procedure involving DEAE-cellulose chromatography and elution from a blue Sepharose column and the hypothesis that glucagon regulates pyruVate Kinase activity, at least in part, by a phosphorylation mechanism is supported.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-02-10 and is currently open access. It has received 107 citations till now. The article focuses on the topics: Pyruvate kinase & Pyruvate dehydrogenase kinase.read more
Citations
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Molecular physiology of the regulation of hepatic gluconeogenesis and glycolysis.
S J Pilkis,Daryl K. Granner +1 more
TL;DR: It is clear that PEPCK mRNA is stabilized by agents that increase the rate of transcription of the gene, and under appropriate metabolic signals this dual control of mRNA synthesis and stability provides for a long-term increase in PEPCk mRNA and protein.
Journal ArticleDOI
Role of calcium in the hormonal regulation of liver metabolism
Journal ArticleDOI
Phospholipid-sensitive Ca2+-dependent protein kinase from heart. II. Substrate specificity and inhibition by various agents.
B. C. Wise,D. B. Glass,C. H. J. Chou,Robert L. Raynor,N. Katoh,R C Schatzman,R S Turner,Robert F. Kibler,J.F. Kuo +8 more
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Gluconeogenesis in teleost fishes
Raul K. Suarez,Thomas P. Mommsen +1 more
TL;DR: The literature on the substrates, enzymes, pathway, regulation, and physiological function of gluconeogenesis in teleost fishes is reviewed and it is suggested that the third task can be achieved only through the integration of an isolated enzyme to whole organism.
Journal ArticleDOI
Hepatic Gluconeogenesis/Glycolysis: Regulation and Structure/Function Relationships of Substrate Cycle Enzymes
S J Pilkis,T H Claus +1 more
TL;DR: The results obtained allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
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A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate
TL;DR: A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase.
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Crystallization, Characterization and Metabolic Regulation of Two Types of Pyruvate Kinase Isolated from Rat Tissues
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Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach.
Leslie D. Ryan,Carl S. Vestling +1 more
TL;DR: A new affinity column has been developed for the isolation of lactate dehydrogenase from rat liver and from two Morris hepatomas, and LDH of maximum specific catalytic activity and single-component behavior during polyacrylamide electrophoresis can be obtained in high yield within a period of several hours, depending on the scale of the preparation.