Structure of the octopine synthase upstream activator sequence
TLDR
A transcriptional activating element within the 5' flanking sequence of the Agrobacterium tumefaciens octopine synthase (ocs) gene that is necessary for ocs expression in transformed tobacco calli is identified.Abstract:
We have identified a transcriptional activating element within the 5' flanking sequence of the Agrobacterium tumefaciens octopine synthase (ocs) gene that is necessary for ocs expression in transformed tobacco calli. This element is located between 333 and 116 base pairs upstream from the transcription initiation site and functions independent of orientation when placed upstream of the ocs gene. It does not function in either orientation when placed downstream of the gene, nor can it activate its promoter when separated by a distance of 608 base pairs. Deletion analysis indicates that sequences essential for activator function are localized between 222 and 177 base pairs upstream of the transcription initiation site. Another region, located between 333 and 249 base pairs upstream of the transcription initiation site, does not as a monomer activate the ocs promoter, but it can as a dimer.read more
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Phenylalanine ammonia-lyase gene organization and structure
C. L. Cramer,K. Edwards,Michel Dron,Xaiowu Liang,Xaiowu Liang,S. L. Dildine,G P Bolwell,Richard A. Dixon,Christopher J. Lamb,Wolfgang Schuch +9 more
TL;DR: Southern blot hybridization of genomic DNA fragments revealed three divergent classes of PAL genes in the bean genome, and polymorphic forms were observed within each class.
Journal ArticleDOI
An octopine synthase enhancer element directs tissue-specific expression and binds ASF-1, a factor from tobacco nuclear extracts.
TL;DR: In vivo expression patterns and in vitro binding properties of the ocs palindromic sequence are remarkably similar to those of the as-1 element of the cauliflower mosaic virus 35S promoter, suggesting the involvement of ASF-1 in the transcriptional regulation of the Ocs promoter and the 35S promoters.
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Nopaline synthase promoter is wound inducible and auxin inducible.
TL;DR: In this article, the authors demonstrate that the nos promoter is wound inducible in both vegetative and reproductive organs, suggesting that the response is not organ specific. But the wound response was further enhanced by addition of auxins.
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Novel Plant Transformation Vectors Containing the Superpromoter
TL;DR: Novel plasmids and T-DNA binary vectors are developed that incorporate a modified and more useful form of the superpromoter that was tested in stably transformed tobacco and maize plants and in transiently transformed maize Black Mexican Sweet protoplasts.
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Designing of an artificial expression cassette for the high-level expression of transgenes in plants
TL;DR: The results substantiate the functional validity of the features identified and demonstrate the potential of computational biology in designing artificial expression cassettes for applications in biotechnology.
References
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DNA cloning : a practical approach
TL;DR: The construction and characterization of vaccinia virus recombinants expressing foreign genes Bovine papilloma virus DNA: A eukaryotic cloning vector is studied.