Journal ArticleDOI
Studies on colony formation in vitro by mouse bone marrow cells. II. Action of colony stimulating factor
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An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures in vitro, finding that colony formation was shown to be related to the concentration and not the total amount of CSF.Abstract:
An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures in vitro. Colony formation was shown to be related to the concentration and not the total amount of CSF. The concentration of CSF determined the rate of new cluster initiation in cultures and the rate of growth of individual clusters. Colony growth depleted the medium of CSF suggesting that colony cells may utilise CSF during proliferation. Bone marrow cells incubated in agar in the absence of CSF rapidly died or lost their capacity to proliferate and form clusters or colonies. CSF appears (a) to be necessary for survival of cluster-and colony-forming cells or for survival of their proliferative potential, (b) to shorten the lag period before individual cells commence proliferation and (c) to increase the growth rate of individual clusters and colonies.read more
Citations
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Journal ArticleDOI
Established cell lines of mouse marrow adherent cells producing differentiation factor(s) for the granulocyte-macrophage lineage.
TL;DR: Two continuous cell lines derived from long-term cultures of AKR mouse bone marrow adherent cells release colony stimulating activity (CSA), a factor that induces in vitro differentiation of granulocyte-macrophage progenitor cells, which closely compares with the CSA from MLCM in terms of the populations of colony and cluster forming cells stimulated.
Book ChapterDOI
The Control of Neutrophil and Macrophage Production at the Progenitor Cell Level
TL;DR: This chapter will briefly review the current knowledge on the regulation of the formation of neutrophils and monocyte-macrophages in semisolid in vitro cultures.
Journal ArticleDOI
RAPID CHANGES IN NUCLEOSIDE TRANSPORT INDUCED BY GROWTH INHIBITORS Studies with Neoplastic Mast Cells
C A Lingwood,D. Brian Thomas +1 more
TL;DR: Aqueous extracts of murine embryonic or uterine tissue, or [6N]O2'-dibutyryl 3',5'-adenosine monophosphate (dbc-AMP) which were cytostatic for the murine mastocytoma P815Y in vitro also induced rapid changes in the incorporation of exogenous nucleosides into acid-insoluble material.
Journal ArticleDOI
Cellular responsiveness to stimulation in vitro: increased responsiveness to colony stimulating factor of bone marrow colony-forming cells treated with surface-active agents and cyclic 3'5' AMP.
W. A. Fleming,T. A. McNeill +1 more
TL;DR: Addition of low concentrations (10 ng/ml) of saponin or Tween 80 to stimulated cultures of normal mouse bone marrow in agar increased the number of granulocyte‐macrophage colonies which developed.
Journal ArticleDOI
Presence of colony promoting activity (CPA) in the supernatant of the long-term bone marrow cultures.
TL;DR: Colony promoting activity (CPA) was detected in the supernatants of the bone marrow cell cultures and CPA-responsive cells (target cells) were found in the bone stem cell cultures as well as in the freshly isolated bone marrow.
References
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Journal ArticleDOI
The growth of mouse bone marrow cells in vitro
TR Bradley,Donald Metcalf +1 more
TL;DR: A simple in vitro technique for the growth of colonies from single cell suspensions of mouse bone marrow involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layer.
Journal ArticleDOI
Stimulation by normal and leukemic mouse sera of colony formation in vitro by mouse bone marrow cells
TL;DR: Using a modification of the agar gel method for bone marrow culture, serum from various strains of mice has been tested for colony stimulating activity and all colonies were initially mainly granulocytic in nature but later became pure populations of mononuclear cells.
Journal ArticleDOI
Stimulation of mouse bone marrow colony growth in vitro by conditioned medium.
T. R. Bradley,Margaret A Sumner +1 more
Journal ArticleDOI
Analysis of colonies developing in vitro from mouse bone marrow cells stimulated by kidney feeder layers or leukemic serum
TL;DR: Cell colonies developing in agar cultures from mouse bone marrow cells following stimulation either by neonatal kidney cell feeder layers or AKR lymphoid leukemia serum were mixtures of granulocytic and mononuclear cells.
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Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.
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