Journal ArticleDOI
The growth of mouse bone marrow cells in vitro
TR Bradley,Donald Metcalf +1 more
TLDR
A simple in vitro technique for the growth of colonies from single cell suspensions of mouse bone marrow involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layer.Abstract:
A simple in vitro technique is described for the growth of colonies from single cell suspensions of mouse bone marrow. The system involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layers.read more
Citations
More filters
Journal ArticleDOI
Conditions controlling the proliferation of haemopoietic stem cells in vitro.
TL;DR: A liquid culture system is described whereby proliferation of haemopoietic stem cells, production of granulocyte precursor cells (CFU‐C), and extensive granulopoiesis can be maintained in vitro for several months.
PatentDOI
Primary bioassay of human tumor stem cells
TL;DR: A bioassay method for supporting human tumor stem cell colony growth is disclosed in this article, which is suitable for culture of a variety of neoplasms of differing histopathology.
Journal ArticleDOI
The human hematopoietic colony-stimulating factors
Steven C. Clark,Robert Kamen +1 more
TL;DR: These advances have led to a much better understanding of the role of the myeloid growth factors in regulating hematopoiesis in vivo that should soon find practical application in clinical medicine.
Journal ArticleDOI
Differentiation and Proliferation of Hematopoietic Stem Cells
TL;DR: This simple model fits the understanding of the interactions of growth factors with hematopoietic progenitors and risks oversimplification of a very complex process.
Journal ArticleDOI
Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells
Lawrence M. Souza,Thomas C. Boone,Janice Gabrilove,Por H. Lai,Krisztina M. Zsebo,Murdock Douglas C,Vicki R. Chazin,Joan Bruszewski,Hsieng Sen Lu,Kenneth Chen,Jean M. Barendt,Erich Platzer,Malcolm A.S. Moore,Roland Mertelsmann,Karl Welte +14 more
TL;DR: Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities, which has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic cancer.
References
More filters
Journal ArticleDOI
A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow Cells
TL;DR: Counts of macroscopic splenic colonies were used to obtain an estimate of the radiation sensitivity of normal mouse bone marrow progenitor cells.
Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
Journal ArticleDOI
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
TL;DR: Application of the methods described in this paper as a tool for quantitative study of normal mammalian cell growth, physiology, genetics, and biochemistry, and the response of cells to drugs, viruses, high energy radiation, and other agents have been indicated.
Journal ArticleDOI
Role of the kidney in erythropoiesis.
TL;DR: Observations in the laboratory indicate that the dynamic equilibrium of the erythron is controlled by the relationship of oxygen supply in the tissue to the demand for oxygen rather than by either alone.
Journal ArticleDOI
Nigrosin as a dye for differentiating live and dead ascites cells.
TL;DR: A simple method is given for modifying ascites cells, i.e., modifying the cell membrane so that it becomes permeable to large molecules such as nigrosin.