Journal ArticleDOI
Studies on colony formation in vitro by mouse bone marrow cells. II. Action of colony stimulating factor
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An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures in vitro, finding that colony formation was shown to be related to the concentration and not the total amount of CSF.Abstract:
An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures in vitro. Colony formation was shown to be related to the concentration and not the total amount of CSF. The concentration of CSF determined the rate of new cluster initiation in cultures and the rate of growth of individual clusters. Colony growth depleted the medium of CSF suggesting that colony cells may utilise CSF during proliferation. Bone marrow cells incubated in agar in the absence of CSF rapidly died or lost their capacity to proliferate and form clusters or colonies. CSF appears (a) to be necessary for survival of cluster-and colony-forming cells or for survival of their proliferative potential, (b) to shorten the lag period before individual cells commence proliferation and (c) to increase the growth rate of individual clusters and colonies.read more
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References
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Journal ArticleDOI
The growth of mouse bone marrow cells in vitro
TR Bradley,Donald Metcalf +1 more
TL;DR: A simple in vitro technique for the growth of colonies from single cell suspensions of mouse bone marrow involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layer.
Journal ArticleDOI
Stimulation by normal and leukemic mouse sera of colony formation in vitro by mouse bone marrow cells
TL;DR: Using a modification of the agar gel method for bone marrow culture, serum from various strains of mice has been tested for colony stimulating activity and all colonies were initially mainly granulocytic in nature but later became pure populations of mononuclear cells.
Journal ArticleDOI
Stimulation of mouse bone marrow colony growth in vitro by conditioned medium.
T. R. Bradley,Margaret A Sumner +1 more
Journal ArticleDOI
Analysis of colonies developing in vitro from mouse bone marrow cells stimulated by kidney feeder layers or leukemic serum
TL;DR: Cell colonies developing in agar cultures from mouse bone marrow cells following stimulation either by neonatal kidney cell feeder layers or AKR lymphoid leukemia serum were mixtures of granulocytic and mononuclear cells.
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