Journal ArticleDOI
Studies on Streptomyces griseus protease. I. Separation of DFP-reacting enzymes and purification of one of the enzymes.
TLDR
In this paper, a commercial preparation of Streptomyces griseus protease (Pronase, Calbiochem) has been shown to contain at least three DFP-reacting components.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1968-02-05. It has received 44 citations till now. The article focuses on the topics: Streptomyces griseus & Esterase.read more
Citations
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Journal ArticleDOI
Virus-Receptor Interaction in an Adenovirus System
TL;DR: Cellular fractionation revealed that receptors were confined mainly to the plasma membrane fraction and that negligible receptor activity could be demonstrated in enzyme-treated cells, and adsorption studies indicate that 10(5) to 10(6) receptor sites are available for the structural protein.
Journal ArticleDOI
Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1.8 A resolution. A model for serine protease catalysis.
TL;DR: In this paper, the 1.8 A resolution structure of the microbial enzyme Streptomyces griseus protease A in its native conformation, and in complexes with Ac-Pro-Ala-pro-Phe-OH (I), Ac Pro-AlA-ProTyr-OH(II) and Ac Pro Ala-ProPhe H (IV), all at pH 4.1.
Journal ArticleDOI
Isoelectric focusing in layers of granulated gels. II. Preparative isoelectric focusing.
TL;DR: Experiments on a quartz plate followed by densitometric evaluation in situ at 280 nm have demonstrated that it is possible to proceed from analytical thin-layer isoelectric focusing to preparative separations without loss of resolution, just by changing the dimension of the gel layer and increasing the protein load.
Book ChapterDOI
Comparative specificity of microbial proteinases.
TL;DR: The hydrolysis of peptides by pepstatin-sensitive enzymes was markedly accelerated by elongating the peptide chain by three amino acid residues on both sides of the splitting point, while the elongation of two residues produced the same effect with the pepStatin-insensitive enzyme.
References
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Journal ArticleDOI
Automatic recording apparatus for use in the chromatography of amino acids.
Journal ArticleDOI
The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin.
TL;DR: It is considered simpler and more useful to describe completely the procedures as they are now used in this laboratory, and to avoid confusion about results already published no radical changes have been made.
Journal ArticleDOI
A modified ninhydrin reagent for the photometric determination of amino acids and related compounds
Stanford Moore,William H. Stein +1 more
TL;DR: A modified ninhydrin reagent for the photometric determination of amino acids and related compounds and its application in drug discovery is described.
Journal ArticleDOI
A spectrophotometric determination of trypsin and chymotrypsin
George W. Schwert,Yasuo Takenaka +1 more
TL;DR: A sensitive spectrophotometric assay for trypsin and chymotrypsin using synthetic substrates has been described.